| PLP-组氨酸脱羧酶的纯化与晶体制备 |
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| 引用本文： | 雷景迈,ML Hackert. PLP-组氨酸脱羧酶的纯化与晶体制备[J]. 华中科技大学学报(医学版), 1989, 0(4) |
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| 作者姓名： | 雷景迈 ML Hackert |
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| 作者单位： | 同济医科大学基础医学部生化教研室(雷景迈)，德克萨斯洲立大学化学系(ML Hackert) |
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| 摘 要： | 本文成功地改进了依赖磷酸吡哆醛的组氨酸脱羧酶(PLP-Histidine Decarboxylase;PLP-HDCase)的纯化方法。通过采用离子交换层析,减少浓缩及透析次数,方法简便。同时,增加Aminohexyl-sepharose层析柱床体积,使一次获得纯化PLP-HDCase量增加10～15倍。在获得足够优质PLP-HDCase的基础上,找到了稳定结晶及晶体生长条件,首次获得大单晶。
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| 关 键 词： | 组氨酸脱羧酶 离子交换层析 结晶 |
The Purification and Crystal Preparation of Morganella Pyridoxal-5-Phosphate Histidine Decarboxylase from E Coli GMI/PGV37 |
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| Lei Jingmai. The Purification and Crystal Preparation of Morganella Pyridoxal-5-Phosphate Histidine Decarboxylase from E Coli GMI/PGV37[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1989, 0(4) |
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| Authors: | Lei Jingmai |
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| Affiliation: | Lei JingmaiDepartment of Biochemistry,Faculty of Basic Medical Sciences,Tongii Medical University,WuhanML Hackert Department of Chemistry LIT of TX Austine |
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| Abstract: | This paper described a modified method of purifying the Morganella pyridoxal-5-phosphate histidine decarboxylase (PLP-HDCase). The yield of PLP-HDCase once increased 10 to 15 times by using only DEAE-cellulose column and Aminohexyl-sepherose column, and the time of purification was reduced. Besides by obtaining sufficient stable crystal condition big crystal was established. Asa result , a big crystal was obtained for the first time. |
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| Keywords: | histidine decarboxylase ion-exchange chromatography crystalization |
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