| 亚砷酸联合LY294002对人鼻咽癌CNE细胞的抑制作用 |
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| 引用本文： | 杨甫文,;伍娟英,;黄慧芳,;陈贤明.亚砷酸联合LY294002对人鼻咽癌CNE细胞的抑制作用[J].中国耳鼻咽喉头颈外科,2014,21(10):515-519. |
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| 作者姓名： | 杨甫文 ;伍娟英 ;黄慧芳 ;陈贤明 |
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| 作者单位： | [1]南京军区福州总医院耳鼻咽喉头颈外科,福建福州350025; [2]福建省血液病研究所福建省血液病学重点实验室福建医科大学附属协和医院,福建福州350000 |
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| 摘 要： | 目的 探讨亚砷酸（As2O3）联合PI3K/Akt信号通路抑制剂LY294002对人鼻咽癌细胞株CNE（简称CNE细胞）的抑制作用。方法 应用MTT法检测CNE细
胞对As2O3和LY294002的敏感性，Western blot方法检测p-PTEN、p-AktSer473、p-AktThr308、p-PDK1等信号分子的表达水平。结果 As2O3与LY294002均可显著抑制CNE细胞的生长，而且该作用呈现明显的剂量依赖性和时间依赖性。As2O3与LY294002的联合应用对CNE细胞生长的抑制作用具有协同效应。LY294002（10 μmol/L）处理4 h即可使CNE细胞内p-AktSer473、p-AktThr308、p-PDK1蛋白表达下调，p-PTEN蛋白表达上调。As2O3（4 μmol/L）单独作用12 h后，CNE细胞内蛋白p-AktSer473表达下调，p-AktThr308、p-PDK1、p-PTEN蛋白变化不明显。LY294002（10 μmol/L）与As2O3（4 μmol/L）联合处理12 h，CNE细胞内p-AktSer473、p-AktThr308、p-PDK1等信号分子的下调水平与p-PTEN蛋白的上调强度显著强于LY294002或As2O3的单独作用。结论 As2O3联合LY294002对CNE细胞具有协同杀伤作用。
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| 关 键 词： | 细胞 培养的 鼻咽肿瘤 细胞增殖 亚砷酸盐类 PI3K/Akt信号通路 |
Inhibitory effects of arsenic trioxide combined with LY294002 on human nasopharyngeal carcinoma CNE cells |
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| Institution: | YANG Fuwen, WU Juanying, HUANG Huifang, CHEN Xianming(1 Department of Otolaryngology Head and Neck Surgery, Fuzhou General Hospital of Nanjing Command, Fuzhou, Fujian, 350025, China; 2 Fujian Insitute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhon, Fujian, 350000, China) |
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| Abstract: | OBJECTIVE To investigate the inhibitory effects of arsenic trioxide（As2O3） combined with PI3K/Akt signal pathway inhibitors LY294002 on the growth of human nasopharyngea1 carcinoma CNE cell lines. METHODS MTT assays were used to determine the sensitivity of CNE cells to As2O3 and LY294002. The expression of p-AktSer473, p-AktThr308, p-PDK1、p-P T E N was detected by Western blot. RESULTS The proliferation inhibitory effects by As2O3 or by LY294002 were time and dose-dependent. As2O3 combined with LY294002 presented a synergistic effect. After treatment with LY294002（10 μmol/L） for 4 h, the expression of p-AktSer473, p-AktThr308 and p-PDK1 protein were significantly down-regulated in CNE cell, and p-PTEN protein was significantly up-regulated. After treat with As2O3（4 μmol/L） for 12 h, the expression of p-AktSer473 protein was significantly down-regulated in CNE cell, and there was no change in p-AktThr308, p-PDK1 and p-PTEN expression. After treatment with As2O3（4 μmol/L） combined with LY294002（10 μmol/L） for 12 h, the expression of p-AktSer473, p-AktThr308 and p-PDK1 protein were significantly down-regulated in CNE cell, and p-PTEN protein was significantly up-regulated. CONCLUSION The specific PI3 K inhibitors LY294002 combined with As2O3 might have synergistic killing effects on human nasopharyngeal carcinoma. |
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| Keywords: | Cells Cultured Nasopharyngeal Neoplasms Cell Proliferation Arsenites PI3K/Akt signal pathway |
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