羟基磷灰石／魔芋葡甘聚糖复合材料的制备和表征

目的：制备羟基磷灰石／魔芋葡甘聚糖复合材料，分析两者间的结合机制以及羟基磷灰石和魔芋葡廿聚糖复合性能最佳时的工艺条件。方法：实验于2007-03／06在昆明理工大学组织工程支架与多孔催化载体实验室完成。通过共沉淀法制备羟基磷灰石（hydroxyapatite，HAP）／魔芋葡甘聚糖（konjac glucomannan，KGM）复合材料。对不同pH样品进行X射线衍射仪物棚分析。KBr压片法制样，傅里叶转换红外光谱仪测定样品的傅里叶转换红外光谱。样品表面喷金后，用扫描电镜观察样品的形貌和微观结构及进行电子能谱分析。结果：复合材料中的无机相为部分碳酸根取代的呈弱结晶状态的HAP，KGM分子链上乙酰基中的羰基是HAP的成核位点。pH值是影响HAP和KGM间交互作用的关键因素，HAP和KGM的原料比对复合性能有明显影响。当pH=9．0和10．7、原料比为w（HAP／KGM）=30／70时，HAP和KGM复合良好。结论：实验制备的HAP／KGM复合材料是一种潜在的骨组织工程支架材料，pH值和HAP／KGM原料比是影响HAP和KGM复合性能的重要影响因素。

Preparation and characterization of hydroxyapatite/konjac glucomannan composites

AIM： To prepare the hydroxyapatite （HAP）/konjac glucomannan （KGM） composite materials, analyze the integrating mechanism of HAP and KGM, and explore the optimal technique condition for the best performance. METHODS： The experiment was carded out in the Laboratory of Tissue Engineering Scaffolds and Porous Catalytic Carders from March to June in 2007. HAP/KGM composites were synthesized by the coprecipitation method. X-ray diffraction analyzer was used for difficult pH values of samples. Sampling was conducted by using KBr pellet method, and detected by using fourier transform infrared spectroscopy. After the samples were metallized by spraying on surface, scanning electron microscope was employed to characterize the composites, including morphology, microstructure and electron spectrum. RESULTS： The inorganic phase in composites was partially carbonate-substituted HAP with low crystallinity, and the carbonyl groups of acetyl groups on the surface of KGM molecules were the nucleation sites of HAP. The pH value was a key influencing factor for the phase interaction between HAP and KGM, and the raw material ratio of HAP/KGM was also important to determine the composite performance of HAP/KGM composites. The two phases of HAP and KGM combined well while pH values were 9.0 and 10.7, the raw material ratio of HAP/KGM was 30/70. CONCLUSION： This prepared HAP/KGM composites will be a potential biomaterial as bone tissue engineering scaffold, and the composite performance can be influenced by the pH value and the raw material ratio of HAP/KGM.