柱前衍生化RP-HPLC测定铁皮石斛鲜品中的氨基酸的含量

目的测定铁皮石斛鲜品中氨基酸的含量。方法样品以异硫氰酸苯酯（PITC）为衍生化试剂衍生后进行HPLC分析。采用CAPCELLPAKC18SG300（250ram×4．6mm，51a,m）色谱柱，柱温为250（：；流动相A为0．05mol／L醋酸钠缓冲液（以醋酸调pH值至6．5）-乙腈（1：1），流动相B为0．05mol／L醋酸钠缓冲液（以醋酸调pH值至6．5），梯度洗脱，流速为0．6ml／rain；检测波长为254nm。结果15种氨基酸浓度在0．077-2．042μg范围内呈良好的线性关系（r：0．9994-0．9999），平均回收率为94．3％-107．7％。结论本方法灵敏、准确，具有良好的重复性和稳定性，可用于铁皮石斛中氨基酸的检测。

Determination of Amino Acids in Dendrobium Officinale Fresh Product with Pre - column derivatization RP - HPLC.

Objective To determine and analyze amino acids in Dendrobium officinale Fresh Product. Methods The sample was derivatized with phenyi isothiocyanate （ PITC）. HPLC was per - formed on a CAPCELL PAK Clg SG300 （ 4.6ram x 250ram, 5 μm） column with gradient elution of 0, 05mol/L sodium acetate buffer solution （ adjusted to pH 6.5 with acetic acid） - acetonitrile （ 1： I ） （A） and 0.05mol/L sodium acetate buffer solution（ adjusted to pH 6.5 with acetic acid） （B） at the flow rate of 0.6ml/min,deteeted at 254nm. Column temperature was 25~C. Results Fifteen aminoacids had good linearity in the ranges of 0. 077 -2. 042p, g （r ：0. 9994 -0. 9999）.The average recoveries were 94. 3% - 107. 7%. Conclusion The method is sensitive and accurate with good stability,which is useful for the determination of amino acids in Dendrobium officinale.