大鼠急性肾损伤后自噬相关蛋白Beclin-1的表达

目的研究自噬相关蛋白Beclin-1在大鼠急性肾损伤伤后肾组织中的表达情况，并探讨其意义。方法选用24只雄性大鼠sD大鼠，体重250±30g，按随机数字表法分为两组，假手术组（Sham组）和缺血-再灌注组（I／R组）；各组于再灌注后12、24、48h3个相应时间点收获肾脏标本。HE染色观察肾脏病理变化，免疫组化法测定自噬相关蛋白Beclin-1的表达情况，透射电镜观察。肾脏组织自噬现象。结果与假手术组比较，大鼠急性肾损伤48h，肾小管细胞Beclin-1蛋白的表达最强（P〈0．05），再灌注损伤后48h，自噬体数量显著增多，细胞内出现染色质块状边集，核形态不规则的明显凋亡现象。结论大鼠急性肾损伤后自噬相关蛋白Beclin-1的表达上调，说明大鼠肾急性。肾损伤后肾脏组组织自噬活性上调。

Expression of Autophagy - associated Protein Beclin - 1 after Acute Kidney Injury in Rats.

Objective To study the expression of autophagy - associated protein Beclinl and its significance in the kidney tissue af- ter ischemia - reperfusion injury in rats. Methods The acute kidney injury was induced by occlusion in Sprague - Dawley （SD） rats. The rats were randomly divided into sham - operated group and ischemia - reperfusion group. The reperfusion was performed at 30 min. The expression of Beclin - 1 was analyzed by immunohistochemistry. Autophagy was evaluated by transmission electron microscopy. Re- sults Compared with the sham - operated group, the expression level of Beclin 1 reached a peak at 48h after ischemia - reperfusion （ P 〈 0.05）. Autophagy body significantly increased 48h after reperfusion. Conclusion AKI induced by ischemia - reperfusion injury leads the up - regulation of Beclin - 1 in rat, which demonstrates up - regulation of autophagic activity in rat kidbey after ischemia and reperfusion.