阳离子脂质体介导的Ａ20基因转染人外周血单核细胞的可行性

目的探讨阳离子脂质体介导的A20基因转染人外周血单核细胞的可行性及最佳转染条件。方法采用脂质体LipofectamineTM2000包裹pCAGGS-GFP／A20质粒转染人外周血单核细胞，通过荧光显微镜检测GFP报告基因，RT-PCR检测A20基因表达。结果应用LipofectamineTM2000介导的A20基因转染人外周血单核细胞，在2×10 6／L细胞接种浓度，脂质体与质粒的量之比为4μl：3μg时，转染效果最佳，RT-PCR证实成功获得A20目的基因的表达。结论脂质体转染法可介导A20基因在人外周血单核细胞获得表达，该研究为A20基因转染的相关实验研究提供参考依据。

Feasibility of Human Peripheral Blood Mononuclear Cell Transfected by Cationic Liposome Mediated A20 Gene.

Objective The feasibility and the optimal transfection conditions of human peripheral blood mononuclear of cationic lipo- some mediated A20 gene transfeetion were investigated in this paper. Methods Human peripheral blood mononuclear cells were trans- fected by pCAGGS - GFP/A20 plasmid encapsulated by liposome Lipofectamine TM 2000, GFP reporter gene was detected by fluorescence microscopy expression of A20 gene was detected by RT - PCR. Results Transfectiun effeet was hest when cells inoculation concentration was 2 x 106/1, and liposome and plasmid ratio was 4μl： 3μg by applying Lipofectamine TM 2000 mediated A20 gene to transfeet blood mononuelear cells of human peripheral. RT - PCR confirmation successfully achieved the expression of A20 objective gene. Conclusion The method of liposome transfection mediated A20 gene in human peripheral blood mononuciear cells was expressed. Such study provides a reference for related experimental study of A20 gene transfection.