普通肝素管代替无菌肝素管制备染色体的可行性分析

目的:探讨普通肝素管能否代替无菌肝素管进行染色体制备。方法:分别用普通肝素管和无菌肝素管随机抽取20例体检者的外周血,同步培养,经培养、秋水仙素处理、收获、低渗、固定、滴片、染色等环节后镜检,并对结果比较分析。结果:普通肝素管和无菌肝素管制备成功率均为100﹪,镜下两种标本中期分裂相整体观无明显差异(P>0.05),两种标本中期分裂相数目比较无显著性差异(P>0.05)。结论:普通肝素管和无菌肝素管制备结果无明显差异,可使用普通肝素管代替无菌肝素管用于染色体制备。

The feasibility analysis the ordinary heparin tube instead the sterile heparin tube preparation preparation of chromosome

Objective： To investigate the ordinary heparin tube can substitute for heparin tube for chromosome preparation. Methods： By using ordinary heparin pipe and aseptic heparin respectively, 20 peripheral blood specimen from Volunteers examination were randomly selected, synchronous culture these two specimens, colchicine treatment, harvest, low permeability, fixed, microscopy after drop, dyeing, and the data was statistically analyzed. Results：Success rate of both ordinary heparin tube and sterile heparin method was 100 % , under the microscopic, no difference of specimens overall view existed between the two method during the middle split phase, there was no statistically significant difference between the number in middle split phase of the two specimen（P〉0.05）. Conclu sion： There is no statistically significant difference between regular heparin tube and aseptic controls for heparin during chromosome preparation. Can use ordinary heparin tube instead of sterile heparin tube to use for chromosome preparation.