多囊卵巢综合征患者血清中PEDF和超敏C反应蛋白的表达及其相关性研究

目的研究色素上皮衍生因子（PEDF）和超敏c反应蛋白（hs—CRP）在多囊卵巢综合征（PCOS）患者血清中的表达变化，并探讨二者相关性。方法选择40例BMI≤25kg／m2的PCOS患者（PCOSl组）和40例BMI〉25ks／m。的PCOS患者（PCOS2组）；另选BMI匹配的健康女性各30例，分为对照1组和对照2组。分离血清后，采用酶联免疫吸附法检测PEDF，采用免疫比浊法检测hs-CRP；应用SPSSl4．0对各组数据进行统计学分析。结果与对照组相比，PEDF和hs-CRP在PCOS患者血清中均明显增高，且与正常体质量的PCOSl组相比，hs-CRP水平在肥胖的PCOS2组进一步显著增高。相关性分析显示，PEDF与hs-CRP呈正相关。结论血清PEDF水平与hs-CRP密切相关，揭示代谢紊乱与亚临床慢性炎症共同促进PCOS病情的发展。

Relationship of Serum Pigment Epithelium - Derived Factor and High Sensitive C - reactive Protein in Women with Polycystic Ovary Syn~drome.

Objective To investigate the serum levels of pigment epithelium - derived factor （PEDF） and high sensitive C - reac- tive protein （hs - CRP） and to examine their relationship in polycystic ovary syndrome （PCOS） women. Methods Forty PCOS women with ≤25kg/m2（PCOS 1 group） and 40 PCOS women with BMI 〉 25kg/m2 （PCOS 2 group） were recruited. We also selected 60 BMI - matched healthy controls, 30 in control 1 group and 30 in control 2 group. ELISA was done to detect serum PEDF ; immunoturbi- dimetry was done to detect serum hs - CRP. Correlation analysis was conducted by using software SPSS 14.0. Results Compared to con- trols groups, PEDF and hs - CRP in PCOS groups were significantly increased （P 〈 O. 05 ）. What＇s more, hs - CRP in PCOS 2 group washigher than in PCOS 1 group （ P 〈 O. 05 ）. PEDF was positively related to hs - CRP; r = O. 452 in PCOS1 and r = O. 478 in PCOS2 （ P 〈 O. 05 ）. Conclusion PEDF in PCOS women was closely related to hs - CRP, which indicated that metabolic disturbance and sub - clini- cal chronic inflammation together contribute to the progress of PCOS.