Spasmolytic activity of Rosmarinus officinalis L. involves calcium channels in the guinea pig ileum |
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Authors: | Ventura-Martínez Rosa Rivero-Osorno Oscar Gómez Claudia González-Trujano María Eva |
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Institution: | a Departamento de Farmacología, Facultad de Medicina, UNAM. Av. Universidad 3000, Ciudad Universitaria, Coyoacán, C.P. 04510, México, DF, Mexico b Laboratorio de Neurofarmacología de Productos Naturales, Dirección de Investigaciones en Neurociencias del Instituto Nacional de Psiquiatria “Ramón de la Fuente Muñiz”, Calz. México-Xochimilco 101, Col. San Lorenzo Huipulco, C.P. 14370, México, DF, Mexico |
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Abstract: | Ethnopharmacological relevanceRosmarinus officinalis L. is a plant used around the world for its properties to cure pain in several conditions, such as arthritic and abdominal pain or as an antispasmodic; however, there are no scientific studies demonstrating its spasmolytic activity. Therefore, the aim of the present study was to investigate the effect of an ethanol extract from Rosmarinus officinalis aerial parts and the possible mechanism involved by using rings from the isolated guinea pig ileum (IGPI).Materials and methodsThe IGPI rings were pre-contracted with potassium chloride (KCl; 60 mM), acetylcholine (ACh; 1 × 10−9 to 1 × 10−5 M) or electrical field stimulation (EFS; 0.3 Hz of frequency, 3.0 ms of duration and 14 V intensity) and tested in the presence of the Rosmarinus officinalis ethanol extract (150, 300, 600 and 1 200 μg/mL) or a referenced smooth muscle relaxant (papaverine, 30 μM). In addition, the possible mechanism of action was analyzed in the presence of hexametonium (a ganglionic blocker), indomethacine (an inhibitor of prostaglandins), l-NAME (a selective inhibitor of the nitric oxide synthase) and nifedipine (a calcium channel blocker).ResultsRosmarinus officinalis ethanol extract exhibited a significant and concentration-dependent spasmolytic activity on the contractions induced by KCl (CI50 = 661.06 ± 155.91 μg/mL); ACh (CI50 = 464.05 ± 16.85 μg/mL) and EFS (CI50 = 513.72 ± 34.13 μg/mL). Spasmolytic response of Rosmarinus officinalis (600 μg/mL) was reverted in the presence of nifedipine 1 μM, but not in the presence of hexamethonium 0.5 mM, indomethacine 1 μM or l-NAME 100 μM.ConclusionThe present results reinforce the use of Rosmarinus officinalis as antispasmodic in folk medicine. Moreover, it is demonstrated the involvement of calcium channels in this activity, but not the participation of nicotinic receptors, prostaglandins or nitric oxide. |
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Keywords: | Calcium channels Folk medicine Guinea-pig ileum Rosmarinus officinalis L Spasmolytic |
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