组织工程皮肤用真皮支架细胞相容性的比较研究

目的研究几种真皮支架的细胞相容性，为构建组织工程皮肤提供资料。方法取胶原海绵、明胶海绵、PEGA和PHBV共4种真皮支架，与小鼠成纤维细胞和ES源表皮样干细胞体外培养，观察前者对后者粘附率的影响，及对成纤维细胞增殖的影响，评价真皮支架的细胞相容性。结果成纤维细胞和ES源表皮样干细胞在4种支架材料上均能粘附存活，成纤维细胞能增殖，但在胶原海绵、PHBV和PLGA支架上的增殖率明显高于明胶海绵。结论几种真皮支架的细胞相容性较好，可用于新型组织工程皮肤的构建。

Comparative Study on Cell Compatibility of Dermal Scaffolds in vitro

Objective To investigate the cell compatibility of materials for dermis and to provide data for tissue engineering skin.Methods Four scaffolds which including collagen sponge,gelatin sponge, Poly lactic acid_co_glycolic acid(PLGA) and poly 3_hydroxybutyric acid_co_3_hydroxyvaleric acid(PHBV) were prepared and cocultured with fibroblast or ES_derived epidermal stem cells in vitro.The cell compatibility was estimated through the adhesion rate and proliferation rate to fibroblast and the adhesion rate to ES_derived epidermal stem cells.Result Fibroblast and ES_derived epidermal stem cells all could adhere and survive in the four porous scaffolds and the fibroblast could proliferate,and the proliferation rate were higher in collagen sponge,PHBV and PLGA than in gelatin sponge.Conclusion The cell compatiblility is good in the four dermal scaffolds and suitable for constructure the new tissue engineering skin.