P物质在诱导肉芽组织成纤维细胞增殖中的作用

目的　探讨感觉神经肽P物质 (substanceP ,SP)对离体培养的肉芽组织成纤维细胞的促增殖作用及其对碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)基因表达的调控作用。　方法　采用MTT法测定SP对原代培养的肉芽组织成纤维细胞的促增殖作用 ;采用RT PCR方法检测SP对成纤维细胞bFGF基因表达的调控作用 ,观察时间及剂量 效应关系。　结果　 10 -9～ 10 -5mol/L的SP在体外对原代培养的肉芽组织成纤维细胞均具有明显的促增殖作用 (P <0 0 1) ,且具有明显的剂量依赖性 (r=0 5 94 ,P <0 0 1) ,bFGF抗体能部分抑制这一作用。在作用后 3、6hSP可诱导成纤维细胞bFGFmRNA的表达 ,在 10 -9～ 10 -5mol/L范围内均可以显著促进成纤维细胞bFGFmRNA表达 ,在 10 -7mol/L达到峰值 (P <0 0 1)。　结论　SP对肉芽组织成纤维细胞具有明显的促增殖作用 ,这种作用与其诱导内源性bFGF基因表达有关

Effects of substance P on granulation tissue fibroblasts proliferation and expression of basic fibroblast growth factor mRNA

Objective To explore the proliferation promoting effect of sensory neuropeptide substance P (SP) on the cultured granulation tissue fibroblasts in vitro and its regulative effect on the gene expression of basic fibroblast growth factor (bFGF) mRNA. Methods The proliferation promoting effect of cultured granulation tissue fibroblasts was observed by means of MTT; the regulative effect of SP on gene expression of fibroblast bFGF by RT PCR. The time and dose efficiency relations were also observed. Results There was a significant proliferation promoting effect of SP on the cultured granulation tissue fibroblasts in vitro in a remarkable dose dependent fashion. However, bFGF antibody only partly exerted its inhibitive effect. SP could induce the bFGF mRNA expression of the fibroblasts at the 3rd and 6th hour ( P < 0 01). SP could promote the bFGF mRNA expression of the fibroblasts in the concentration of 10 -9 -10 -5 mol/L and peaked in the concentration of 10 -7 mol/L. Conclusions SP has a significant proliferation promoting effect on the granulation tissue fibroblasts, which is correlated with SP inducing bFGF mRNA expression of fibroblasts.