| 枳实提取液对豚鼠心室肌细胞L型钙电流的影响 |
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| 摘 要： |
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| 关 键 词： | 枳实 提取液 豚鼠 心室肌细胞L型钙电流 影响 中药 |
Effect of Citrus aurantium extract on L-type calcium currents in ventricular myocytes of single guinea pigs] |
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| Authors: | Fang Fang Min Dong Hong Zhu |
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| Institution: | Laboratory of Molecular Pharmacology, Xiangya School of Medicine, Central South University, Changsha, 410078, China. |
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| Abstract: | OBJECTIVE: To determine the effect of citrus aurantium extract (CAE) on L-type calcium currents (ICa-L) in ventricular myocytes of guinea pigs. METHODS: Single myocytes were dissociated by enzymatic dissociation method. The whole-cell patch-clamp recording technique was used to record the change of calcium current after the administration of CAE. RESULTS: 1. CAE 4 x 10(-3), 2 x 10(-2), 4 x 10(-2), and 1 x 10(-1) g/ml increased the ICa-L by 15.89%, 29.01%, 52.03% and 59.76% (P < 0.05), respectively. 2. CAE 2 x 10(-1) and 4 x 10(-1) g/ml decreased the ICa-L by 16.61% and 30.87% (P < 0.05), respectively. CAE changed the ICa-L significantly, but did not change the shape of I-V curves. CONCLUSIONS: CAE 4 x 10(-3)-1 x 10(-1) g/ml can increase the L-type calcium current in guinea pig ventricular myocytes significantly in a concentration-dependent manner, and promote the opening of calcium channel. CAE 2 x 10(-1)-4 x 10(-1) g/ml may inhibit the L-type calcium channels of ventricular myocytes, and depress the opening of calcium channel. |
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