结缔组织生长因子及抗体对体外培养鸡滑膜细胞增殖活性的影响

目的：研究不同浓度结缔组织生长因子（CTGF）及其抗体对鸡肌腱滑膜细胞增殖活性的影响.方法：以来亨鸡屈趾肌腱腱周滑膜组织为材料,经体外分离、培养和鉴定后,加入不同浓度的CTGF（浓度为0,5,10,20,50,100,200,500 ng/ml）及CTGF抗体（浓度为0,1,2,4,8,16,32,64 μg/ml）分组培养滑膜细胞,并以CCK-8染色法检测滑膜细胞的增殖情况.结果：滑膜细胞增殖在CTGF浓度为5 ng/ml至200 ng/ml时,呈逐步递增趋势,各组之间差异有统计学意义（P＜0.05）;而在200 ng/ml及500 ng/ml浓度组间差异无统计学意义（P＞0.05）,加入浓度为4,8,16,32 μg/mlCTGF抗体时,滑膜细胞增殖呈逐步递减趋势,各组之间差异有统计学意义（P＜0.05）;而CTGF抗体浓度为0,1,2 μg/ml和浓度为32,64μtg/ml时,各组间差异无统计学意义（P＞0.05）.结论：CTGF可促进体外培养的鸡肌腱滑膜细胞增殖,最佳浓度为200 ng/ml;CTGF抗体对滑膜细胞增殖活性可产生一定程度的抑制作用.

The effect of connective tissue growth factor and its antibody on proliferation activity of tendon synovial cells in vitro

Objective：To investigate the effect of connective tissue growth factor （CTGF） and its an- tibody on proliferation activity of tendon synovial cells. Methods：The tendon synovial cells were ob- tained from leghorn tendon synovial tissues and cultured with different concentration of CTGF and its antibody. Cell Counting Kit-8 was used to detect proliferation activity of synovial cells. Results： The proliferation activity of synovial cells increased gradually as the concentration of CTGF eleva- ting from 5 ng/ml to 200 ng/ml, significant tration （P〈0.05）. However, no significant ly di higher than that in the control group at each concen fference was observed between synovial cells cultured with 500 ng/ml CTGF and those cultured with 200 ng/ml CTGF. And the proliferation acti synovial cells decreased gradually as the concentration of CTGF antibody elevating from 4 μg vtty /ml of to32 μg/ml, significant and those activity of significantly lower than that in the control group at each concentration （P〈0. 05）. no difference was observed between synovial cells cultured with 64 μg/ml CTGF antibody cultured with 32μg/ml CTGF antibody. Conclusion. CTGF can promote the proliferation synovial cells in vitro with a optimal concentration of 200 ng/ml. And CTGF antibodycan inhibit the proliferation activity of synovial cells .