人血清中万古霉素浓度的改良监测方法

目的：优化HPLC测定人血清中万古霉素浓度的方法.方法：在不同流动相配比、沉淀剂及柱温条件下,高效液相色谱法测定血万古霉素浓度,确定最佳的色谱条件和操作方法.结果：在色谱条件为色谱柱C18（250.0 mm×4.6 mm,5μm）、流动相磷酸二氢钾缓冲液（pH 3.2）-乙腈（90：10,v/v）、流速1.0 ml/min、检测波长236 nm、柱温35℃的情况下,万古霉素浓度在4.47～60.83 mg/L范围内线性关系良好（r=0.9 943,n=5）,回收率在85％～115％范围内;日内、日间RSD＜15％,在定量下限附近RSD＜20％.结论：优化后,操作简便快速,回收率稳定,适于临床血药浓度测定.

The improved monitoring and determination for serum vancomycin concentration

Objective： To optimize the method for the determination of serum vancomycin concentra tion by HPLC. Methods： Chromatographic peaks of serum vancomycin concentration under differ- ent mobile phase, precipitant and column temperature were analyzed to find the best cbromato graphic condition and operation method. Results： The better linearity range of vancomycin concen- tration was between 4.47 mg/L and 60.83 mg/L（r=0. 9 943, n=5）by using the hypersil C18 （250.0 mm×4.6 mm,5μm）, potassium dihydrogen phosphate（pH3. 2）-acetonitrile （90 ： 10, v/ v） as mobile phase, flow rate 1.0 ml/min, detection wavelength 236 nm and column temperature 35℃, and the recovery rate was at therange of 85% to 115%. The RSD values of intra-day and in ter-day were lower than 15%, and the RSD values of LLQ lower limit of quantification were lower than 20 %. Conclusion： The improved method is simple and rapid, and the recovery rate is stable It is suitable for the application of clinical and scientific research.