青蒿琥酯联合阿霉素对人舌鳞癌Tca8113细胞抑制作用的实验研究

目的研究青蒿琥酯(Art)与阿霉素(ADM)联合应用对人舌鳞癌Tca8113细胞增殖的影响。方法通过MTT法测定Art和ADM对Tca8113细胞增殖的抑制作用;应用流式细胞术分析细胞周期变化;利用吖啶橙-溴化乙锭(AO-EB)染色观察经Art作用前后Tca8113细胞形态的改变;应用透射电镜观察药物对细胞超微结构的影响。结果 Art和ADM对Tca8113有显著地抑制增殖和诱导凋亡作用,并呈明显的时间-剂量效应(P0.05);Art(25μg.mL-1)和ADM(0.39～1.56μg.mL-1)联合给药可对Tca8113产生增强的联合杀伤效果(P0.01);流式细胞术检测Art可使细胞周期阻滞于G2/M期和S期;透射电镜和荧光染色观察可见凋亡细胞染色质凝聚、边集呈新月形。结论 Art对Tca8113细胞有显著地抑制作用,其机制可能为阻滞细胞周期于G2/M期和S期以及诱导细胞凋亡。Art与ADM合用具有较好联合效应,可增强Tca8113对ADM的敏感性。

Experimental Research of the Inhibition of the Combined Treatment of Artesunate with Adriamycin on Tongue Squamous Cell Carcinoma Tca8113

Objective To study the impacts of artesunate（Art）combined with adriamycin（ADM）on anti-proliferation of tongue squamous cell carcinoma Tca8113.Methods MTT method was used to determine the inhibition of Tca8113 proliferation treated with combined Art and ADM.The flow cytometry（FCM）was applied to analyze the changes in cell cycle.AO-EB staining was adopted to observe the morphologic changes in Tca8113 before and after Art administration.Transmission electron microscopy was applied to observe the impact of drugs on ultrastructure of cell.Results Art and ADM inhibited Tca8113 proliferation and induce apoptosis significantly,indicating apparent time-dose dependence（P0.05）.The combination of Art（25 μg·mL-1）and ADM（0.39~1.56 μg·mL-1）showed the enhanced joint-killing effect on Tca8113（P0.01）.It was detected with FCM that the cell cycle was blocked at G2/M phase and S phase.Transmission electron microscopy and fluorescent staining found chromatin condensation and crescent-shaped edge of apoptotic cell.Conclusion Art has significant inhibition on Tca8113,probably due to the cell cycle blockage at G2/m phase and S phase and the induction of apoptosis.The co-administration of Art and ADM presents satisfactory joint effect,which may enhance the sensitivity of Tca8113 to ADM.