3q27-3q29相关的p63蛋白表达在弥漫性大B细胞淋巴瘤中的意义

 目的　探讨3q27-3q29相关的p63蛋白表达在弥漫性大B细胞淋巴瘤（DLBCL）中的意义。方法　应用免疫组织化学EnVision二步法检测102例DLBCL与15例反应性淋巴组织增生（RHL）中p53蛋白和3q27-3q29相关的p63蛋白的表达并进行随访。结果　p53、p63蛋白在DLBCL中的阳性率分别为62 %和56 %，在RHL中的阳性率分别为0和13 %，p53、p63蛋白在DLBCL与RHL中比较差异均有统计学意义（P＜0.05）；p53、p63蛋白的表达在Ⅰ+Ⅱ期中的阳性率分别为48.3 %和41.4 %，在Ⅲ+Ⅳ期中的阳性率分别为79.5 %和75 %，差异有统计学意义（P＜0.05）；p53、p63蛋白的表达在生发中心B细胞型（GCB型）中的阳性率分别为28 %和28 %，在非GCB型（non-GCB型）中的阳性率分别为72.7 %和64.9 %，差异均有统计学意义（P＜0.05）。p53、p63蛋白表达与患者性别、年龄、有无B症状和发病部位等无相关性（P＞0.05）。p53蛋白表达与p63蛋白表达呈正相关性（r＝0.629，P＜0.05）。p53、p63蛋白表达阴性组的5年总体生存率高于阳性组（38 %∶6 %，51 %∶4 %），差异有统计学意义（P＜0.05）。结论　p63可能作为癌基因与p53共同参与了DLBCL的发生和发展，两者在肿瘤的发生中可能起着协同作用；联合检测p63蛋白和p53蛋白在DLBCL中的表达，可能成为判断DLBCL预后指标之一。

Significance of 3q27-3q29-related p63 protein expression in diffuse large B-cell lymphoma

Objective To investigate the expressions and clinicopathological significance of 3q27-3q29-related p63 protein in diffuse large B-cell lymphoma (DLBCL). Methods An immunohistochemical Envision~(TM) method was used to detect the expressions of p53 and 3q27-3q29-related p63 protein in 102 cases of DLBCL and 15 cases of reactive hyperplasia of lymph node (RHL). Results The tumor cell expressions of p53(62 %) and p63(56 %) in DLBCL were significantly higher than that in RHL (0 and 13 % P < 0.05). The expressions of p53 and p63 were significantly different (1) between stage Ⅰ + Ⅱ (the positive rate 48.3 % and 41.4 %, respectively) and stage Ⅲ+Ⅳ(the positive rate 79.5 % and 75 %, respectively; P <0.05), (2) between GCB type (the positive rate 28 % and 28 %, respectively) and non-GCB type(the positive rate 72.7 % and 64.9 %, respectively; P <0.05). The expressions of p53 and p63 had no relationship to gender, age, B symptoms and locations. The expression of p53 was positively correlated with that of p63 in DLBCL (P <0.05, Cp=0.629). p53 and p63 protein expression in negative group the 5-year overall survival rate is higher than that in positive group (38 % and 6 %, 51% and 4 %, respectively), the difference was statistically significant (P <0.05). Conclusion It was likely that p63, as the oncogene, participated in the occurrence and development of DLBCL together with p53. Also, p63 and p53 might play a synergistic role in the occurrence DLBCL. Combined detection of 3q27-3q29-related 1963 protein and p53 protein in DLBCL, might be one of the indicators to the prognosis of DLBCL.