Epidermal growth factor and basic fibroblast growth factor: effects on an overlapping population of neocortical neurons in vitro |
| |
Authors: | Harley I Kornblum Heather K Raymon Richard S Morrison Kathleen P Cavanaugh Ralph A Bradshaw Frances M Leslie |
| |
Institution: | Department of Pharmacology, College of Medicine, University of California, Irvine 92717. |
| |
Abstract: | Epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF) have trophic effects on rat neocortical neurons in vitro. Concentration-response studies reveal that EGF maximally stimulates neuronal survival and process outgrowth at approximately 10 ng/ml, while the maximal effect of bFGF is seen at 10-30 ng/ml. Treatment with maximal concentrations of bFGF results in cultures containing a greater number of neurons with long processes, as well as greater branching of processes, than does treatment with EGF. When EGF and bFGF are added together to cultures the effects are not additive. In addition, bFGF is capable of supporting the survival of neurons previously treated with EGF. These findings indicate that EGF and bFGF affect a largely overlapping population of neocortical neurons, but that bFGF may be a more effective trophic agent for these cells. |
| |
Keywords: | Epidermal growth factor Basic fibroblast growth factor Cerebral cortex Neuronal morphology Neurotrophic factor Cell culture |
本文献已被 ScienceDirect 等数据库收录! |