补锌对缺锌大鼠烧伤后肠道细菌移位及毒血症的影响

目的:探讨补锌对大鼠烧伤后肠道细菌移位及毒血症的影响。方法:90只SD大鼠进食低锌饲料(含锌量:1.6ug·g-1)1周,造成缺锌模型,并随机分为正常对照组(A)、烧伤对照组(B)、烧伤治疗组(C)。B组和C组造成20%总体表面积Ⅲ度烧伤。B组伤后继续进食低锌饲料,C组伤后进食正常含锌饲料(含锌量:24.7ug·g-1)。采用甲基-3H胸腺嘧啶核苷(3H]-TdR)标记致病大肠杆菌,在烧伤前注人大鼠的胃肠道。在不同的时象点,检测血浆内毒素(LPS)含量、血和肝的3H放射活性。结果:(1)B、C组3H的放射活性和血浆LPS含量显著高于A组(P<0.05);同时,C组显著低于B组(P<0.05)。(2)B、C组肝的3H放射活性比A组显著增高(P<0.05),C组比B组低。结论:烧伤后补锌能减轻大鼠肠道细菌移位及毒血症。

The effect of zinc supplements on enteral bacterial translocation and toxemia in rats after burn

Objective： To investigate the effect of zinc supplements on enteral bacterial translocation and toxemia in rats after burn. Method： Ninety Spregue-Dawley rats were pair-fed with zinc deficient diets （zinc content： 1.6μg. g^-1） for one week to induce zinc deficient status and were randomly divided into 3 groups, i. e. normal control （A）, burn control （B） and treatment （C） groups. Rats in B and C groups were subjected to a 20% TBSA full-thickness thermal injury. Rats in B group were fed with zinc deficient diets （zinc content： 1.6μg. g^-1） after injury and rats in C group fed with normal zinc content diets （zinc content： 24. 7μg. g^-1） after injury. Pathogenic E. Coli labeled with methylated 3H-thymidine was infused into experimental rats＇ intestine before injury. At different time points, plasma lipopolysaccharide （LPS） was determined. 3H radioactivity was assayed. Result： （1）. Blood 3H radioactivity and plasma LPS level in B and C groups were much higher than that in A group （P＜0. 05）. Simultaneously, those in C group were much lower than those in B group （P＜0. 05）. （2）. 3H radioactivity in the rats ＇ liver in B and C groups was much higher than that in A group （P＜0. 05）. Conclusion： Zinc could decrease enteral bacterial translocation and lessen toxemia in rats after burn.