流式细胞术细胞因子分析法检测乙型肝炎患者特异性细胞免疫功能的初步研究

目的运用流式细胞术胞内细胞因子（CFC）分析法检测乙型肝炎患者外周血特异性细胞免疫功能，探讨该技术检测乙型肝炎病毒（HBV）特异性细胞免疫功能的临床应用价值。方法采用HBV核心抗原表位肽core18-27设计CFC分析法，检测急、慢性乙型肝炎患者外周血单个核细胞（PBMC）中γ-干扰素（IFN-γ）分泌细胞的数量。结果19例急性乙型肝炎、54例慢性乙型肝炎患者PBMC中IFN-γ分泌细胞的数量差异有统计学意义（P〈0．01）。结论CFC分析法能在体外直接检测乙型肝炎患者PBMC中IFN-γ分泌细胞的数量，其水平在一定程度上反映了机体特异性细胞毒性T淋巴细胞应答状况。急性乙型肝炎患者特异性细胞免疫功能明显强于慢性乙型肝炎患者，

Preliminary study on the detection of specific cellular immunity of patients with hepatitis B by cytokine flow cytometry

Objective To evaluate the clinical application of cytokine flow cytometry （ CFC ） for testing the specific cellular immunity of patients with hepatitis B and to investigate the difference of cellular immunity in patients with various types of hepatitis B. Methods The number of γ-interferon（IFN-γ） secreting cell in peripheral blood mononuclear cell （PBMC） in samples of acute and chronic hepatitis B was tested by CFC and HBV core18-27 peptide. Results The number of IFN-γ secreting cell was significantly different between 19 patients with acute hepatitis B and 54 patients with chronic hepatitis B （P 〈0. 01 ）. Conclusions CFC is a direct in vitro method to detect the number of IFN-γ, secreting cells which reflects the response of cytotoxic T lymphocyte. The specific cellular immunity is stronger in patients with acute hepatitis B than in patients with chronic hepatitis B.