羧甲基壳聚糖对大鼠胃溃疡抗氧化作用研究

目的:探讨羧甲基壳聚糖对大鼠乙酸型胃溃疡的保护作用及可能机制。方法:将40只SD大鼠随机分为正常组、模型组、硫糖铝组和羧甲基壳聚糖组。用20%乙酸0.05ml浆膜下注射法建立大鼠慢性胃溃疡模型,手术后第3天开始灌胃给药,连续14天后计算各组溃疡面积,同时检测胃粘膜组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:羧甲基壳聚糖、硫糖铝治疗后,乙酸诱导的溃疡面积缩小;羧甲基壳聚糖能提高大鼠胃粘膜SOD活性,抑制MDA的升高,其作用与硫糖铝相当。结论:羧甲基壳聚糖对大鼠乙酸型胃溃疡有一定保护作用,可能与它能提高胃粘膜抗氧化能力有关。

Study on the antioxidant activity of Carboxymethyl-chitosan in gastric ulcer rats

Objective: To investigate the protective role and its influential factors of Carboxymethyl-chitosan(CM-Chitosan) against acetic acid-induced chronic gastric ulcers in rats.Methods: 40 Sprague-Dawley rats were randomly divided into normal group,model group,sucralfate group and CM-chitosan group.The rat models of chronic gastric ulcers were established by injecting 20% acetic acid 0.05ml into gastric mucosa.14 days after the administration,rats were killed.The ulcer area was measured,and the contents of superoxide dismutase(SOD) and malondialdehyde(MDA) in gastric mucosa were detected.Results: CM-chitosan reduced the ulcer area of ulcer rats induced by acetic acid.CM-chitosan up-regulated the level of SOD in gastric mucosa.The level of MDA in gastric mucosa was decreased in CM-chitosan group.Conclusion: Our results suggest that CM-chitosan has protective effects on rats with acetic acid-induced gastric ulcers.It might reduce gastric ulcer by increasing antioxidant effects on gastric mucosa.