强啡肽[DynA(1-8)]对体外神经细胞老化过程中细胞周期和总蛋白的影响

用小鼠神经母细胞瘤细胞无血清培养建立神经细胞老化实验模型。以流式细胞光度术观察DynA(1-8)对老化过程中神经细胞的细胞周期和细胞总蛋白的影响。结果发现DynA(1-8)可延缓细胞老化的细胞周期和细胞总蛋白的变化。提示DynA(1-8)是通过使细胞周期和细胞总蛋白发生变化以发挥其延缓神经细胞老化的作用。

EFFECTS OF DYNORPHIN A (1-8) ON CELL CYCLE AND TOTAL CELLULAR PROTEIN DURING NEURONAL AGING IN VITRO

Serum-free culture of mouse neuroblastoma cells was usetd as the experimentalmodel for the study of neuronal aging , with flow cytometry of cell cycle , DNA and total cellularprotein as the indices of neuronal aging.After addition of dynorphin A(1-8)10^-7mol·L^-1 into theculture medium, the following general tendencies were obtained:(1) The number of S and G₂+Mphase cells was increased and the number of G_{1 phase cells decreased.(2) The total cellular protein inaged experimental neural cells decreased. The results implies that Dyn A( 1-8) Produced effects oncell cycle, DNA and total cellular protein in the delaying neuronal aging. The relationbetween Dyn A(1-8) and neuronal aging mierits further investigation.}