NGF诱导PC12细胞向神经元的分化

目的：研究NGF诱导PC12细胞向神经元的分化情况。方法：实验分为10、20、50和80 μg?L^-1 NGF组及含10%胎牛血清的对照组。不同浓度的NGF组均诱导PC12细胞72 h，在24、48和72 h分别观察细胞的突起长度和细胞最大直径的变化情况；在诱导72 h后利用免疫细胞化学技术，观察不同剂量NGF作用后PC12细胞向MAP2阳性细胞分化的情况。 结果： 20、50和80 μg?L^-1 NGF组MAP2阳性细胞数明显高于对照组，且以50 μg?L^-1 组最为明显（P<0.05）。与对照组比较，加入NGF组分化的细胞其突起和细胞直径明显增长和增大，以50 μg?L^-1 组最为明显（P<0.01）。结论：NGF能够诱导PC12细胞向神经元分化，50 μg?L^-1 的NGF浓度是诱导PC12细胞向神经元分化的最适浓度。

Differentiation of PC12 cells into neurons induced by NGF

Objective To study the condition of pheochromocytoma cells(PC12 cells)differentiation into neurons induced by nerve growth factor(NGF).Methods There were five groups :10,20,50,and 80 μg·L-1 NGF groups and control group which contained 10% fetal calf serum.PC12 cells were exposed to NGF with different concentrations for 72 h and the lengh of neurite and max diameter of PC12 cells were observed at 24,48,and 72 h,respectively.The expression of MAP2 was detected by immunocytochemistry after PC12 cells were exposed to NGF for 72 h.Results The number of MAP2 positive cells was significantly increased in 20,50,and 80 μg·L-1 groups compared with control group and the most significant concentration was 50 μg·L-1(P<0.05).The length of total primary neurite and max diameter of PC12 cells in 20,50,and 80 μg·L-1 NGF groups were obviously longer and bigger than those in control group and the most significant concentration also was 50 μg·L-1(P<0.01).Conclusion PC12 cells could differentiate into neurons induced by NGF,and 50 μg·L-1 is the optimal concentration in which NGF could induce PC12 cells to differentiate into neurons.