调心方对AD模型鼠脑组织呼吸链氧化酶活性和氨基酸递质含量的影响

为研究调心方(主药：党参、桂枝、茯苓、蕾蒲、远志)治疗阿尔茨海默病(AD)的机理．将SD雄性大鼠脑窒内注射DHF-FeCl3-ADP,造成氯化损伤型类AD模型(造模前1周起以调心方灌胃．每日1次．连续4周);采用Clark氯电极法观察鼠脑线粒体呼吸链氯化酶活性：HPLC-荧光法观察鼠脑氨基酸递质含量;RT-PCR法观察大脑皮质NMDAR(NR1a和NR2a)mRNA表达．以及调心方对AD大鼠上述指标变化的影响．并与Donspezil治疗组比较。结果：模型大鼠脑组织氨基酸递质含鼠和NMDAR(NR1a和NR2a）mRNA表达高于正常对照组。而线粒体呼吸链细胞色素氯化酶活性则显着下降;调心方对上述各项指标有不同程度的纠正作用。提示调心方可以通过纠正AD模型鼠脑组织呼吸链氯化酶活性和氨基酸递质的异常变化．这可能是该方防治AD的机理之一。

Influence of "Heart-Regulating Decoction" on Respiration-Chain Oxidase Activity and Amino Acid Transmitter in Brain Tissue in AD Rats

To study the mechanism of "Heart-Regulating Decoction" in the treatment of Alzheimer's disease (AD), SD male rats were modeled to be of AD by intracerebral injection of DHF-FeCl 3-ADP. Before modeled, "Heart-Regulating Decoction" was given by gastric perfusion, once a day and for 4 weeks. The respiration-chain oxidase activity, amino acid transmitter and the mRNA expression of NMDAR were measured; moreover, Donepezil was used as control. Results: In model group, the content of amino acid transmitter and the mRNA expression of NMDAR increased, higher than those in normal group, while the mitochondrion respiration-chain cytochrome oxidase reduced obviously; and the Decoction could correct them to different extents. It is indicated that "Heart-Regulating Decoction" can correct the abnormal changes in the respiration-chain oxidase activity and amino acid transmitter, this may be one of its mechanisms in the prevention and treatment of AD.