α－干扰素对外周血单个核细胞中HBVDNA的清除作用

用聚合酶链反应（ＰＣＲ）方法动态观来了α－干扰素治疗的６例慢性乙型病毒性肝炎患者外周血单个核细胞（ＰＢＭＣ）与血清中乙型肝炎病毒（ＨＢＶ）ＤＮＡ的清除情况。血清ＨＢＶＤＮＡ在治疗５周后（３－１０周）６例转阴，其中２例在结束治疗时复转阳性，ＰＢＭＣ中ＨＢＶＤＮＡ在治疗１５周（１２－２０周）时５例转阴，１例始终阳性，并出现ＡＬＴ复升高。结果显示α－干扰素能清除ＰＢＭＣ中ＨＢＶＤＮＡ，但滞后于血清病毒的清除，ＰＢＭＣ中ＨＢＶＤＮＡ持续阳性，可能使治疗中期效果较差。

The effect of interferon alpha on hepatitis B virus DNA in peripheral blood mononuclear cells

. R.China Six patients with chronic active hepatitis B virus(HBV)infection were investigated for HBV DNAboth in serum and in peripheral blood mononuclear cells(PBMCs)by polymerase chain reaction(PCR) pri-or,during and after interferon(IFN)alpha treatment. All patients responded for IFN therapy with remis-sion of serum alanine aminotransferase(ALT).HBV DNA both in serum and in PBMCs disappeared in 6cases and 5 cases respectively during treatment of interferon alpha.The average elimination period of HBVDNA was 5 weeks (3-10 weeks)in serum and 15 weeks(12-20 weeds)in PBMCs.Elevation of serumALT activity was seen in one patient with HBV DNA in PBMCs during and after treatment of IFN alpha.The results showed that clerance of HBV DNA in PBMCs was more unlikelv than tbat in serum,and thatpermenant appearance of HBV DNA in PBMCs during and after treatment of IFN alpha may imply thefailure of IFN therapy.