中药循经敷贴对哮喘豚鼠肺组织Eotaxin mRNA和IL-5 mRNA表达的影响

目的探讨中药循经敷贴治疗哮喘的作用机制。方法采用Hutson法豚鼠模型，治疗组隔日诱喘后用中药敷贴30min；对照组隔日诱喘后采用生理盐水浸润纱布敷贴，时间同治疗组。记录各组诱喘潜伏期时间。2周后处死豚鼠，取肺组织，用半定量RT-PCR法比较Eotaxin mRNA、IL-5 mRNA的表达。结果中药循经敷贴能显著延长豚鼠诱喘潜伏期，下调哮喘豚鼠肺组织Eotaxin mRNA、IL-5 mRNA的表达。结论抑制变异性炎症可能是中药循经敷贴治疗哮喘的重要机制之一。

Effect of herbal penetration therapy on Eotaxin mRNA, IL-5 mRNA expression in lung tissue of guinea pigs with experimental allergic asthma

AIM To investigate the mechanism of external application of Chinese herbs on allergic asthma. METHODS The asthma guinea pig models induced by Huston＇ s methods were externally applied with Chinese herb for 30 min, qod. A control group of guinea pigs were applied with wet gauzes in 9 g· L^- 1 normal saline in same times . The arousal time was compared and signed. The lung tissues were got after they were killed 2 weeks later. The method of RT- PCR was used to compare the expression of Eotaxin mRNA and IL-5 mRNA. RESULTS The arousal time could be prolonged significantly and the expression of Eotaxin mRNA and IL-5 mRNA could be fallen through the external application of Chinese herbs. CONCLUSION The anti-inflammation factors may be the one of the important mechanism of herbal penetration therapy on allergic asthma.