HPLC法测定人血清中硫酸头孢噻利浓度

目的建立一种灵敏、可靠的测定人血清中硫酸头孢噻利浓度的高效液相色谱二极管阵列检测方法。方法血清样品经酸化甲醇蛋白沉淀后，以0．02mol·L-1乙酸铵-甲醇（75：25，V/V）为流动相，色谱柱为ExtendC18柱（250mm×4．6mm，5um），头孢唑林为内标，检测波长为254m，流速为0．5mL·min-1。结果硫酸头孢噻利的绝对回收率为79．9％-84．7％，方法回收率为91．7％-103．3％，日内精密度（RSD）〈5．2％，日间精密度（RSD）〈7．3％，硫酸头孢噻利质量浓度在0．02-1．0mg·L-1时具有良好线性，定量检出限为0．02mg·L-1。结论本方法具有简便、灵敏、准确等优点，可用于硫酸头孢噻利的临床药动学研究及临床特殊人群的血药浓度测定。

Determination of concentration of cefoselis sulfate in human serum by HPLC

A/M To develop a sensitive and accurate method for the determination of cefoselis sulfate in human serum by high-performance liquid chromatography with diode army detection（DAD） method. METHODS After eefoselis sulfate in human serum was extracted by protein precipitation with acidic methanol, the separation was performed on an Extend Cls column（250 mm ×4.6 mm, 5 tan）using the mobile phase consisted of 0.02 mol.L-1 acetic ammonium＋ methanol （75：25, V/V）. Detection was earried out by DAD at 254 nm with a flow rate of 0.5 mL.min-1. RESULTS Calibration curve was linear within the range of 0.02 - 1.0 mg. L- 1, the absolute recoveries for eefoselis sulfate were from 79.9 % to 84.7%, and the method recoveries were from 91.7 % to 103.3%. The intra-day relative standard deviations （RSDs） were less than 5.2%, and the inter-day RSDs were less than 7.3%. The limit of quantification was 0.02 mg. L- 1 for cefoselis sulfate. CONCLUSION This method is found to be simple, sensitive, accurate for pharmaeokinetie studies and analysis of eefeselis sulfate in human serum for patients.