清胰片质量标准的研究

[目的]建立清胰片(大黄、白芍、胡黄连等)的质量标准.[方法]采用薄层色谱法对大黄、白芍和胡黄连进行定性鉴别;采用高效液相法对黄芩中黄芩苷的含量进行测定.色谱柱为TLANHE-C<,18>(250mm×4.5 mm,10μm),流动相:甲醇-水-磷酸(47∶53∶0.2),检测波长280 nm,流速为1.0 mL/min,柱温为30℃.[结果]薄层色谱法(TLC)法可鉴别出大黄、白芍和胡黄连相对应的斑点,阴性对照无干扰.黄芩苷在0.27～1.08 μg范围内进样量与峰面积呈良好的线性关系,r=0.999 8,平均回收率为99.46%,标准偏差(RSD)为0.39%.[结论]薄层色谱法呈现的斑点清晰,特异性强,可用于清胰片的鉴别,高效液相法可靠、准确,专属性强,重现性好,可用于该制剂质量控制.

Quality standard of QingYi tablet

[Objective] To establish the quality standard of QingYi Tablets (Radix Et RhiZoma Rhei ,Radix Paeoniae Alba, Rhizoma Picrorhizae).[Methods] Radix Et RniZomu Rhei ,Rellix Pheouiae Alba and Rnizoma Picrorhizal were identified by TLC. The content of Baicalin was determined by HPLC. The separation was performed on C18 Column with methanol-Water-Phosphorus acid (47:53:0.2) as a mobile phase. The flow rate was 1.0mL/min, detection Wavelength at 280 nm.[Results] The developed TLC spots were quite clear. The conten...