合格库血与肝炎病毒的传播

目的　探讨合格库血在传播肝炎病毒方面所起的作用。方法　采用ELISA方法及PCR技术对合格库血进行了HBsAg ,抗 HBs ,HBeAg ,抗 HBe ,抗 HBc,抗 HCV ,HBV DNA ,HCV RNA的回顾性检测。 结果　 116 2袋合格库血中HBsAg ,抗 HBs,HBeAg ,抗 HBe ,抗 HBc ,抗 HCV ,HBV DNA ,HCV RNA检出率分别为 :0 .17% (2 / 116 2 ) ,2 7.4(318/116 2 ) ,0 .9% (10 / 116 2 ) ,5 .6 % (6 5 / 116 2 ) ,2 4.3(2 82 / 116 2 ) ,0 .17(2 / 116 2 ) ,15 .0 % (174/ 116 2 ) ,1.5 % (17/ 116 2 ) ,其中在 5 6 3份HBsAg ,抗 HCV全部阴性者中HBV DNA ,HCV RNA检出率分别为 :8.9% (5 0 /5 6 3) ,2 .8% (16 /5 6 3)。 结论　仅以HBsAg ,抗 HCV阴性作为排除HBV ,HCV的感染是欠妥的 ,应增加抗 HBs ,HBeAg ,抗 HBe ,抗 HBc的检测 ,同时采用PCR技术来筛出ELISA全部阴性 ,HBV DNA ,HCV RNA仍阳性的携带者 ,减少输血后肝炎的发生。

Eligible Bank Blood and Transmission of Hepatic Virus

Objective To discuss the action of eligible bank blood on the transmission of hepatic virus. Method Using ELISA and PCR to detect HBsAg, anti HBs, HBeAg, anti HBe, anti HBc, anti HCV, HBV DNA, HCV RNA of eligible bank blood retrospectively. Result Positive rate of HBsAg, anti HBs, HBeAg, anti HBe, anti HBc, anti HCV, HBV DNA, HCV RNA in 1 162 bags of eligible bank blood is respectively 0.17%(2/1 162), 27.4%(318/1 162), 0.9%(10/1 162), 5.6(65/ 1 162), 24.3%(282/1 162), 0.17%(2/1 162), 15.0%(174/1 162), 1.5%(17/1 162), especially positive rate of HBV DNA, HCV RNA in 563 shares of HBsAg and anti HCV negative ones is 8.9%(50/563), 2.8%(16/563) respectively. Conclusion It is improper to exclude HBV, HCV infection only on the basis of HBsAg and anti HCV negative, the detection of anti HBs, HBeAg, anti HBe, anti HBc, should be added, PCR is also useful to screen the carrier in whom all ELISA is negative but HBV DNA, HCV RNA positive for reducing the occurrence of posttransfusive hepatitis.