龟分枝杆菌药物敏感性与耐药性探讨

目的：探讨龟分枝杆菌对抗生素的敏感性和耐药机理。方法：从我市工厂医院内感染标本中分离龟分枝杆菌12株。用琼脂扩散法测定抗生素的敏感性，用滤纸片进行联合药敏试验，并提取、鉴定质粒，用氟哌酸消除质粒。结果：42种抗生素中，3株龟分枝杆菌龟亚种对大多数氟喹诺酮类、氨基糖甙类的抗生素敏感。对抗结核药利福平、吡嗪酰胺、乙胺丁醇敏感。9种脓肿亚种对大多数氨基糖甙类的抗生素敏感，对氟喹诺酮类的环丙沙星敏感，对多数抗结核药耐药。在药物间均为无关作用。从6株脓肿亚种中提取出一个9．1kb质粒，消除质粒后耐药性不变。结论：龟分枝杆菌是一种对多种抗生素耐药的细菌，药物敏感性差异较大。其耐药性与质粒传递无关。

Research of susceptibility and resistance to antibiotics in mycobacterium chelonei

Objective: To investigate the susceptibility to antibiotics and the mechanism of the resistance in Mycobacterium chelonei. Methods: To determine the susceptibility of antibiotics by the method of ag ar dilution and to test the combination drug susceptibility by the method of the filter paper. 12 strains of Mycobacterium chelonei were isolated from cases of the hospital infection in the factory clinics in Chongqing and the plasmids were extracted and eliminated by norfloxacin in these bacteria. Results: In 42 antibiotics, 3 of M. chelonei subspecies were susceptible to most of antibiotics,such as aminoglycosides, fluoroquinolones and rifampin,pyrafat, ethambutol in the antituberculosis agents and 9 of M. Chelonei subspecies abscesses were susceptible to most of antibiotics of aminoglycosides, ciprofloxacin in fluoroquinolones but resistant to antituberculosis agents in the test. The noncorrelation between any 2 antibiotics was observed. A 9.1 Kb plasmids profile was observed in 6 bacteria, but as it was eliminated, the resistance to antibiotics was not c hanged. Conclusion: The bacteria are multiply resistant and have great differences in their susceptibility. The resistance to antibiotics was not translated by plasmid.