| 粮食中3种镰刀菌毒素的气相色谱测定法 |
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| 引用本文： | 温肇霞,杨宗军,宋扬. 粮食中3种镰刀菌毒素的气相色谱测定法[J]. 青岛大学医学院学报, 2001, 37(1): 35-36 |
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| 作者姓名： | 温肇霞 杨宗军 宋扬 |
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| 作者单位： | 青岛大学医学院松山医院内科;青岛市妇幼保健院检验科;青岛大学医学院营养研究所 |
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| 摘 要： | ①目的 建立一种简单、灵敏、高效的测定粮食中雪腐镰刀菌烯醇 (NIV )、脱氧雪腐镰刀菌烯醇(DON)、新茄病镰刀菌烯醇 (NS)的方法。②方法 粮食样品用甲醇 水 (95∶5 ,V∶V)提取 ,经Florisil柱净化 ,用 9∶1的氯仿 甲醇进行洗脱 ,提取液经n 7氟丁酰咪唑衍生后 ,用配有SE 5 4弹性石英毛细管柱的气相色谱进行测定 ,检测器为电子捕获检测器。③结果 建立了粮食中 3种镰刀菌毒素的气相色谱测定方法 ,其最低检测限为 1×10 -11～ 1× 10 -10 g ,各毒素回收率均在 85 %以上。④结论 该方法灵敏、简单、快速、回收率高 ,具有很好的实际应用价值。
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| 关 键 词： | T-2毒素 色谱法 气相 谷物 |
| 文章编号： | 1001-4047(2001)01-0035-02 |
| 修稿时间： | 2000-09-22 |
GAS CHROMATOGRAPHIC METHOD FOR DETECTING THREE KINDS OF FUSARIUM MYCOTOXIN IN GRAINS |
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| WEN Zhaoxia,YANG Zongjun,SONG Yang. GAS CHROMATOGRAPHIC METHOD FOR DETECTING THREE KINDS OF FUSARIUM MYCOTOXIN IN GRAINS[J]. Acta Academiae Medicinae Qingdao Universitatis, 2001, 37(1): 35-36 |
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| Authors: | WEN Zhaoxia YANG Zongjun SONG Yang |
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| Affiliation: | WEN Zhaoxia,YANG Zongjun,SONG Yang Department of Internal Medicine,The Affiliated Songshan Hospital of Qingdao University Medical Colledge,Qingdao 266021, [ |
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| Abstract: | Objective To set up a simple method for detecting three kinds of fusarium mycotoxin in grains. Methods Mycotoxins were extracted with methanol-water(95∶5,V∶V) and purified by chomatographic procedure using Florisil column. The column filters were concentrated and the residue was dissolved in methanol. A portion of the solution was reacted with n-heptaflurobylimidazol and analysed with gas chromatography. Results The limit for detection of the three mycotoxins was 1× 10-11-1×10-10g, and the recoveries were all over 85%. Conclusion This method was simple and highly efficient. |
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| Keywords: | T 2 toxin chromatography gas cereals |
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