| HPLC测定血浆中荷叶碱的浓度及其在大鼠体内的药代动力学研究 |
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| 引用本文： | 刘虹,郝彧,朱晓月,王萌,张德芹.HPLC测定血浆中荷叶碱的浓度及其在大鼠体内的药代动力学研究[J].中国实验方剂学杂志,2010,16(12):165-168. |
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| 作者姓名： | 刘虹 郝彧 朱晓月 王萌 张德芹 |
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| 作者单位： | 天津中医药大学中医药研究院药学部方剂学教育部重点试验室,天津,300193 |
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| 基金项目： | 国家重大科技专项-"重大新药创新" |
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| 摘 要： | 目的: 建立血浆中荷叶碱的HPLC-UV测定方法,研究大鼠灌胃给予荷叶总生物碱提取物后荷叶碱的药代动力学特征。 方法: 采用液液萃取法处理血浆样品,采用HPLC-UV法测定,以盐酸巴马汀为内标,色谱柱为Agilent Zorbax-SB C18(4.6 mm×150 mm, 5 μm),流动相为乙腈-水-三乙胺-冰醋酸(27 ∶70.6 ∶1.6 ∶0.78),流速1.0 mL ·min-1,检测波长270 nm,柱温25 ℃,采用WinNolin软件计算药代动力学参数。 结果: 血浆中荷叶碱在20~1 000 μg ·L-1线性关系良好( r =0.998 5),方法的日内精密度(RSD)2.24%~3.20%,日间RSD为5.54%~6.95%,准确度(RE)为-7.00%~4.25%,提取回收率为87.2%~104%。大鼠灌胃荷叶总生物碱3个剂量(106,212,319 mg ·kg-1)后, t 1/2分别为(4.18±2.50),(4.32±1.01),(6.05±3.72) h, t max分别为(1.50±0.41),(2.25±1.19),(1.88±0.25) h, C max分别为(278.75±38.98),(621.75±137.81),(1 146.50±249.44) μg ·L-1,AUC0→t分别为(1 796.10±680.87) h ·μg -1·L-1,(4 463.49±1 892.42) h ·μg -1·L-1和(7 452.08±3 594.24) h ·μg-1 ·L-1。 结论: 该法灵敏度高,专属性强,准确可靠,操作快速简便,已应用于荷叶碱在大鼠体内的药代动力学研究,阐明了荷叶碱在大鼠体内的药代动力学特征。
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| 关 键 词： | 荷叶总生物碱 荷叶碱 高效液相色谱法 药代动力学 |
| 收稿时间： | 2010/4/16 0:00:00 |
Determination of Nuciferine in Rat Plasma by HPLC and Its Application to Pharmacokinetic Study |
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| LIU Hong,HAO Yu,ZHU Xiao-yue,WANG Meng and ZHANG De-qin.Determination of Nuciferine in Rat Plasma by HPLC and Its Application to Pharmacokinetic Study[J].China Journal of Experimental Traditional Medical Formulae,2010,16(12):165-168. |
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| Authors: | LIU Hong HAO Yu ZHU Xiao-yue WANG Meng and ZHANG De-qin |
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| Institution: | Research Institute of Tianjin University of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medical Formulation in Ministry of Education, Tianjin 300193, China;Research Institute of Tianjin University of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medical Formulation in Ministry of Education, Tianjin 300193, China;Research Institute of Tianjin University of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medical Formulation in Ministry of Education, Tianjin 300193, China;Research Institute of Tianjin University of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medical Formulation in Ministry of Education, Tianjin 300193, China;Research Institute of Tianjin University of Traditional Chinese Medicine, Key Laboratory of Traditional Chinese Medical Formulation in Ministry of Education, Tianjin 300193, China |
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| Abstract: | |
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| Keywords: | alkaloidal extract of N nucifera nucifefine HPLC pharmacokinetics |
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