氯化钆对急性坏死性胰腺炎肺泡巨噬细胞分泌炎症介质的影响

目的 ：探讨氯化钆（Gdcl3）对急性坏死性胰腺炎（ANP）肺泡巨噬细胞 (AM)分泌炎症介质的影响。方法 ：90只成年SD大鼠随机分为正常对照组（C组）、ANP组、Gdcl3处理正常对照组（C+Gdcl3组）、ANP Gdcl3预处理组、ANP Gdcl3治疗组。每组18只。各组大鼠均经支气管肺泡灌洗获取肺泡巨噬细胞，行支气管肺泡灌洗液（BALF）中蛋白含量、肺组织髓过氧化物酶（MPO）水平、AM分泌肿瘤坏死因子α（TNF-a）和一氧化氮（NO）水平分析;行血气分析，检测肺湿/干重比值，并行肺组织病理学检查。结果 ：C+Gdcl3组各指标与正常对照组相比差异无显著性（ P >0.05）。ANP Gdcl3预处理组和ANP Gdcl3治疗组除动脉血氧分压外各项指标显著高于正常对照组，差异有显著性（ P <0.05），但与ANP组相比有降低，差异有显著性（ P <0.05）。结论 ：氯化钆可抑制ANP大鼠AM分泌炎症介质，并减轻ANP所致的肺损伤。

The effect of gadolinium chloride on inflammatory mediators secreted byalveolar macrophages in acute necrotizing pancreatitis rats

Objective To explore the effect of gadolinium chloride(Gdcl_3) on inflammatory mediators secreted by alveolar macrophages(AMs)in acute necrotizing pancreatitis(ANP) rats.Methods Ninty SD rats were randomly into five groups(n=18 in each group): normal control(C) group,ANP group,C+Gdcl_3 treatment(C+A) group,ANPGdcl_3 pretreatment(AP) group,ANPGdcl_3 treatment(AT) group.AMs were obtained by bronchoalveolar lavage.The blood gas the weight ratio of wet/dry lung tissue,the protein content of bronchoalveolar lavage fluids(BALF),the myeloperoxidase(MPO) of lung tissue and secretion of TNFa and NO by AMs were evaluated;histologic examination of lung tissue was performed.Results The parameters mentioned above in C+A group compared with C group showed no statistical significance(P>0.05).Except the blood gas,the indicators mentioned above in AP group and AT treatment group were significantly elevated compared with normal control group(P<0.05);but lewer significantly compared to the ANP group(P<0.05).Conclusions Gdcl_3 can inhibit the secretion of inflammatory mediators of AMs and ameliorate lung injury of ANP.