毛细管气相色谱法测定鱼油脂肪乳注射液中DHA和EPA的含量

目的采用毛细管气相色谱法对鱼油脂肪注射液的DHA和EPA含量进行测量的效果进行研究分析。方法抽取同一批次的鱼油脂肪注射液,采用毛细管气象色谱法对DHA和EPA的含量进行测定,载气选择氦气,流速控制在0.5mL/min,柱温进行程序升温,采用FID检测器,分流比为5：1,进样口温度控制在300℃,进样量为1μL。结果经过仔细研究后笔者发现,被检测的鱼油脂肪注射液中DHA甲酯的浓度在0.216～0.712g/L,EPA甲酯的浓度在0.224～0.768g/L的现象关系非常明显,两种成分的回收率均在99%以上,RSD水平均在0.50%以上。结论采用毛细管气象色谱法对鱼油脂肪注射液的DHA和EPA含量进行测量主要具有操作简单、方便快捷、准确度高、重现性好等特点,可作为今后对该药物的成分含量进行测定的常规方法。

Determination of the DHA and EPA of fish fat emulsion injection by capillary gas chromatography

Objective To analyze the testing effectiveness of determination of the DHA and EPA of fish fat emulsion injection by capillary gas chromatography.Methods The same batch of fish fat emulsion injection was chosen,and the EPA and DHA of it were measured by capillary gas chromatography.The carried gas was helium and its flow rate was 0.5 mL/min.Temperature program and FID detector were applied.The split ratio was 5:1 and the injection port temperature was 300℃ with 1 μL sample size.Results It founded that after review the content of DHA methyl ester of fish fat emulsion injection fell in between 0.216 to 0.712 g/L and the EPA methyl ester was between 0.224 to 0.768 g/L.The recovery rate of two components was above 99% and RSD were all more than 0.50%.Conclusion Determination of the DHA and EPA of fish fat emulsion injection by capillary gas chromatography have the character of operation simple,convenient and efficient,high accuracy and reproducible.It can be a conventional method of the determination of the drug ingredients content in the future.