| 丹酚酸B对大鼠离体心脏缺血再灌注损伤的保护作用 |
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| 引用本文： | 高枫,孙桂波,任小宇,聂媛媛,孙静,秦蒙,孙晓波.丹酚酸B对大鼠离体心脏缺血再灌注损伤的保护作用[J].中国中药杂志,2012,37(3):358-361. |
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| 作者姓名： | 高枫 孙桂波 任小宇 聂媛媛 孙静 秦蒙 孙晓波 |
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| 作者单位： | 1. 中国医学科学院药用植物研究所,北京,100193
2. 辽东学院,辽宁丹东,118003 |
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| 基金项目： | 国家科技支撑计划项目(2008BAI51BO2);国家"重大新药创制"科技重大专项(2009ZX09301-003,2009ZX09502-015);国家科技部项目(2008EG150298) |
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| 摘 要： | 目的:研究丹酚酸B对大鼠离体心脏缺血再灌注损伤的作用及其作用机制。方法:SD大鼠48只,随机分为6组,分别是空白对照组、阳性给药组(维拉帕米150μg.L-1)、丹酚酸B高、中、低剂量组(10,5,2.5 mg.L-1),每组8只。采用Langendorff法灌流离体大鼠心脏,停灌25 min后再灌30 min,造成心肌缺血再灌注损伤模型。于大鼠左心房插入水囊导管,记录丹酚酸B对血流动力学指标的影响,测定不同时间点冠脉流出液中天门冬氨酸氨基转移酶(AST),乳酸脱氢酶(LDH)的活性及心肌组织中超氧化物歧化酶(SOD)活性,丙二醛(MDA)的含量。结果:丹酚酸B不同剂量组(10,5,2.5 mg.L-1),可显著改善缺血再灌注所致大鼠的心功能损伤,减少AST,LDH释放和MDA的产生,增加SOD的活性。结论:丹酚酸B对心肌缺血再灌注损伤具有保护作用,其机制可能与改善心肌舒缩功能,清除氧自由基,减少脂质过氧化反应有关。
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| 关 键 词： | 丹酚酸B 心肌缺血再灌注 心脏血流动力学 心肌酶 |
| 收稿时间： | 3/8/2011 12:00:00 AM |
Protective effect of salvianolic acid B on isolated heart ischemia/reperfusion injury in rats |
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| GAO Feng,SUN Guibo,REN Xiaoyu,NIE Yuanyuan,SUN Jing,QIN Meng and SUN Xiaobo.Protective effect of salvianolic acid B on isolated heart ischemia/reperfusion injury in rats[J].China Journal of Chinese Materia Medica,2012,37(3):358-361. |
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| Authors: | GAO Feng SUN Guibo REN Xiaoyu NIE Yuanyuan SUN Jing QIN Meng and SUN Xiaobo |
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| Institution: | The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;Eastern Liaoning University, Dandong 118003, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China;The Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China |
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| Abstract: | Objective: To study the protective effect and mechanism of salvianolic acid B on isolated heart ischemia/reperfusion injury in rats.Method: Forty-eight SD rats were divided into 6 groups randomly(n=8): the control group,the positive administration group(verapamil 150 μg·L-1),and high,middle and low-dose salvianolic acid B groups(10,5,2.5 mg·L-1).The myocardial ischemia/reperfusion injury model was established using the Langendorff method,re-perusing isolated working hearts for 30 min after ischemia for 25 min.A water-bag catheter was inserted in rat left atrium for recording the effect of salvianolic acid B on hemodynamics indexes-AST,LDH,SOD and MDA.Result: Various group with different doses showed that salvianolic acid B decreased AST,release of LDH and formation of MDA and increased SOD activity.Conclusion: Salvianolic acid B showed a protective effect on myocardial ischemia/reperfusion injury.Its mechanism may be related with improvement of cardiac contractility,cleaning of oxygen free radicals and reduction of lipid peroxidation. |
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| Keywords: | salvianolic acid B myocardial ischemia/reperfusion injury cardiac hemodynamics cardiac enzyme |
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