HPLC-ELSD 同时测定白英中延龄草苷和去半乳糖替告皂苷的含量

目的:建立同时测定白英中延龄草苷(trillin)和去半乳糖替告皂苷(desgalactotigonin)含量的HPLC-ELSD法。方法:采用Diamonsil-C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-10 mmol.L-1醋酸铵溶液52∶48,柱温25℃,流速0.6mL.min-1,进样量20μL,检测器漂移管温度为95℃,氮气流速2.3 L.min-1。结果:延龄草苷和去半乳糖替告皂苷分别在20～200 mg.L-1(r=0.999 8)和10～100 mg.L-1(r=0.999 7)内线性关系良好。延龄草苷的平均回收率为99.4%,RSD为0.90%;去半乳糖替告皂苷的平均回收率为100.3%,RSD 1.1%。结论:该方法准确,重复性良好,适用于白英药材的质量控制。

Simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum by HPLC-ELSD

Objective: To establish a HPLC-ELSD method for simultaneous determination of trillin and desgalactotigonin contents in Solanum lyratum.Method: A Diamonsil C18 column(4.6 mm×250 mm,5 μm) was adopted,with the mobile phase consisting of acetonitrile-10 mmol·L-1 ammonium acetate(52∶48).The temperature was 25 ℃,the flow rate was set at 0.6 mL·min-1,and the sample size is 20 μL.The temperature of drift tubes and gas flow rate of the detector were set at 95 ℃ and 2.3 L·min-1,respectively.Result: With in the linear ranges of 20-200 mg·L-1 and 10-100 mg·L-1,trillin and desgalactotigonin show a good linear relationship.The average recovery was 99.4%(RSD 0.90%) for trillin and 100.3%(RSD 1.1%) for desgalactotigonin.Conclusion: The method is so accurate and easily reproducible that it is suitable for the quality control of S.lyratum medicinal materials.