RP-HPLC法测定麻黄附子细辛汤水煎液中麻黄碱的含量

目的建立测定麻黄附子细辛汤水煎液中麻黄碱含量（以盐酸麻黄碱计）的反相高效液相色谱法。方法色谱柱：Diamonsil-C18柱（200mm×4．6mm）；流动相：乙腈：水（含0．02mol·μL^-1磷酸二氢钾和0．2％三乙胺）（10：90）；流速：1．0mL·min^-1；检测波长：210nm；柱温：27℃。结果样品中盐酸麻黄碱的色谱峰与其他组分完全达到基线分离，回归方程为：y=2229．4854x-22．2885，r=0．9998（n=6），线性范围0．1776—1．3320μg，平均加样回收率为101．45％（RSD=I．9％，n=6）。结论所建立的测定方法快速简便，重现性良好，可用于麻黄附子细辛汤水提物中麻黄碱的含量测定。

Determination of ephedrine in the water decoction of Mahuang-Fuzi-Xixin soup by RP-HPLC

OBJECTIVE To establish a RP-HPLC method for the determination of ephedrine（determined as ephedrine hydrochloride）in the water decoction of mahuang-fuzi-xixin-soup. METHODS The sample was separated on a Diamonsil-C18（200mm × 4.6mm）with the mobile phase consisting of acetonitrile and water（containing 0.02mol·μL^-1 potassium dihydrogen phosphate and 0.2 % triethy lamine） （ 10 ： 90） at a flow rate of 1.0mL·min^-1 The detection wavelength was 210nm, and the column was kept at 27℃. RESULTS Chromatographic separation of ephedrine hydrochloride and the co-existed compounds achieved baseline resolution, y = 2229. 4854x-22.2885, r = 0. 9998 （n = 6）, the calibration was linear in the range of 0.1776-1.3320μg, anti the average recovery of ephedrine hydrochloride was 101.45% （RSD= 1.9%, n = 6） .CONCLUSION The method is rapid, simple and reproducible and can be used for the quantity detection of ephedrine in the water decoction of Mahuang-Fuzi- Xixin-soup.