EFFECT OF HYDROCORTISONE ON LONG-TERM HUMAN MARROW CULTURES

In human long-term marrow cultures a relatively high concentration of hydrocortisone (10⁻⁶ M or more) is necessary for the development and subsequent maintenance of some cellular components in the adherent cell layer. However, such concentrations of hydrocortisone seem to be inhibitory for the production of granulocyte/macrophage progenitor cells (GM-CFC). In further attempts to improve these cultures, therefore, it is important to maintain a balance between a concentration of hydrocortisone which is necessary for the development of a competent adherent layer and the concentration which is inhibitory to the production of GM-CFC.