宫颈癌组织中真核细胞翻译起始因子4E蛋白的表达及其临床意义

目的蛋白水平检测真核细胞翻译起始因子4E(eukaryotic initiation factor 4E,eIF4E)在宫颈癌组织中的表达及其与各临床病理参数的相关性。方法组织来自2005年7月—2012年7月经手术后病理确诊的宫颈鳞状细胞癌(鳞癌)和未分化癌标本各30例(含石蜡标本和小块组织液氮罐保存标本),并选取30例癌旁慢性宫颈炎组织作为对照。采用Western blot和免疫组化SP法检测30例宫颈鳞癌组织、30例宫颈未分化癌组织和30例癌旁慢性炎组织中eIF4E蛋白的表达情况。计量资料采用t检验,计数资料采用χ2P0.05为差异有统计学意义。结果 Western blot检测发现:宫颈鳞癌、未分化癌和癌旁慢性宫颈炎性组织中eIF4E蛋白相对于β-actin的比率分别为0.340±0.017、0.315±0.023和0.122±0.003;免疫组化检测各组织中eIF4E蛋白表达平均吸光度值(MOD值)分别为0.237±0.015、0.219±0.009和0.125±0.037,差异均有统计学意义(P0.05)。分析其与各临床病理参数的相关性发现,宫颈癌临床分期越晚,eIF4E表达率越高;分化程度越高,eIF4E表达率越低;eIF4E表达在淋巴结转移组织中明显高于无淋巴结转移组织(P0.05)。结论 eIF4E与宫颈癌的进展及生物学行为有密切关系,可作为判断宫颈癌恶性程度的指标。

Expression of elF4E protein in cervical cancer and its clinical significance

Objective To investigate the expression of eIF4E （ eukaryotic initiation factor 4E） protein in cervical cancer and its correlation with clinicopathological parameters. Methods From July 2005 to July 2012, 30 tissue samples were from patients with cervical squamous cell carcinoma, and30 from those with cervical undifferentiated carcinoma, both of which were pathologically confirmed after surgery. 30 tissue samples from paraneoplastie tissues of chronic cervicitis were selected as the controls. Immunohistochemistry SP and Western blot methods were used to detect the eIF4E protein levels in all these tissue samples. Results It was found by Western blot that the ratio of eIF4E protein to β- actin was （0. 340 ± 0. 017） in tissues of cervical cancer, （0.315 ±0.023） in tissues of undifferentiated cancer, and （0. 122 ±0. 003） in paraneoplastic tissues of chronic cervicitis. The mean optical densities （MODs） of eIF4E protein were （ 0. 237 ± 0. 015 ）, （0. 219 ±0.009） and （0. 125 ±0. 037）respectively in tissues of undifferentiated cancer, and （0. 122 ±0.003） in paraneoplastic tissues of chronic cervicitis, and the difference was statistically significant（ P 〈 0.05 ）. The analysis of the correlation between eIF4E expression and its clinicopathological parameters showed that the lat r the TNM stage of cervical cancer was, the higher the expression of eIF4E was ; the higher the degree of differentiation was, the lower the expression of eIF4E was; the expression of eIF4E was obviously higher in tissues with lymph node metastasis than in those without（ P 〈 0.05 ）. Conclusions eIF4E has a close relation with the progress and biological behavior of cervical cancer, and can he used as an indicator to determine the degree of malignancy of cervical cancer.