毛细管气相色谱法定量分析地米尿素冰片乳膏中的龙脑和异龙脑

目的:建立地米尿素冰片乳膏中龙脑和异龙脑的定量分析方法。方法:采用毛细管气相色谱法。色谱柱为聚乙二醇20000石英毛细管柱;柱温为140℃;进样口温度为180℃;载气为氮气,流速为1.2mL.min-1;火焰离子化检测器温度为180℃;进样量为1μL。结果:龙脑和异龙脑进样量线性范围分别为113.03～1808.48、86.51～1384.16ng(r=0.9998、0.9991,n=6);平均回收率分别为97.9%和98.2%(RSD=0.8%、0.7%,n=9)。结论:所建立的分析方法快速、准确,适用于地米尿素冰片乳膏中龙脑和异龙脑的定量分析。

Quantitative Analysis of Borneol and Isoborneol in Dexamethasone Urea/Borneol Cream by Capillary GC

OBJECTIVE:To establish a method for quantitative analysis of borneol and isoborneol in Dexamethasone urea/borneol cream.METHODS:Capillary GC was adopted.The determination was carried out on a PEG20000 capillary column.The column temperature was 140 ℃ and using nitrogen as carrier gas at the flow rate of 1.2 mL·min-1.The temperature of hydrogen flame ionization detector was 180 ℃ and injection volume was 1 μL.RESULTS:The linear ranges were 113.03～1 808.48 ng for borneol(r=0.999 8,n=6) and 86.51～1 384.16 ng for isoborneol(r=0.999 1,n=6).Average recoveries were 97.9% and 98.2%(RSD=0.8% and 0.7%,n=9),respectively.CONCLUSION:The method is rapid,accurate and suitable for quantitative analysis of borneol and isoborneol in Dexamethasone urea/borneol cream.