胡萝卜果胶的化学结构特征研究与含量测定

目的:研究胡萝卜果胶的化学结构特征,并测定其含量。方法:采用高效凝胶渗透液相色谱法测定胡萝卜果胶的相对分子质量;纸色谱、高效液相色谱法鉴别果胶中主要单糖组成;紫外、红外光谱法鉴别其化学结构特征;硫酸-咔唑法测定果胶的含量。结果:胡萝卜果胶的平均相对分子质量为1750625,主要单糖组成为D-半乳糖和D-半乳糖醛酸;单糖的连接方式主要为β-吡喃型糖苷键连接。果胶含量以半乳糖醛酸计为66.06%。结论:胡萝卜果胶提取物多糖含量较高、化学结构特征明显。本试验结果可为胡萝卜果胶的进一步开发提供理论和试验基础。

Chemical Structure and Content Determination of Carrot Pectin

OBJECTIVE:To study the chemical structure of carrot pectin and to determine the content of it.METHODS:The average molecular weight was determinate by HPGPC;Paper chromatography (PC) and HPLC were used to identify the major monosaccharide component of pectin;UV and IR spectroscopy indentify the chemical structure;the content of pectin was determined by sulfuric acid-cardyl method.RESULTS:The average molecular weight of carrot pectin was 1 750 625 and monosaccharide mainly composed of D-galactolipin and D-galacturonic acid;monosaccharide was mainly connected by β-pyran type glycosidic bonds.The content of pectin to galacturonic acid was 66.06%.CONCLUSION:The carrot pectin extract has large content of polysaccharide and obvious chemical structure.The trial provides theory and trial basis for further development of carrot pectin.