| 右旋柠烯诱导人胃癌细胞凋亡 |
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| 引用本文： | Lu XG,Feng BA,Zhan LB,Yu ZH. 右旋柠烯诱导人胃癌细胞凋亡[J]. 中华肿瘤杂志, 2003, 25(4): 325-327 |
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| 作者姓名： | Lu XG Feng BA Zhan LB Yu ZH |
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| 作者单位： | 1. 116001,大连医科大学第四临床学院普外科
2. 大连医科大学中医药分子生物学研究室 |
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| 摘 要： | 目的 探讨右旋柠烯 (D limonene)诱导人胃癌细胞株凋亡作用的机制。方法 采用噻唑蓝 (MTT)比色法、电镜、流式细胞术以及免疫细胞化学法 ,对p5 3和bcl 2在肿瘤细胞内的表达以及细胞凋亡的定性与定量指标进行检测。结果 经D limonene处理的BGC 82 3细胞出现核固缩 ,染色质边集 ,凋亡小体形成。凋亡细胞发生率与药物浓度呈正相关 ,即药物浓度为 0 .2 5 μg/ml(4 8h) ,凋亡细胞的发生率从 (2 .71± 0 .78) %上升至 (31.6 2± 7.81) %。经D limonene处理的BGC 82 3细胞内p5 3蛋白表达较对照组明显增加 ,bcl 2蛋白表达较对照组降低。结论 D limonene对人胃癌细胞的杀伤主要是通过诱导细胞凋亡 ,升提p5 3及降低bcl 2的蛋白表达为其诱发肿瘤细胞凋亡的机制之一。
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| 关 键 词： | 胃癌 细胞凋亡 右旋柠烯 p53 bcl-2 |
| 修稿时间： | 2002-12-13 |
D-limonene induces apoptosis of gastric cancer cells |
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| Lu Xiao-guang,Feng Bing-an,Zhan Li-bin,Yu Zhi-hong. D-limonene induces apoptosis of gastric cancer cells[J]. Chinese Journal of Oncology, 2003, 25(4): 325-327 |
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| Authors: | Lu Xiao-guang Feng Bing-an Zhan Li-bin Yu Zhi-hong |
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| Affiliation: | Department of General Surgery, Fourth Hospital of Dalian Medical University, Dalian 116001, China. |
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| Abstract: | Objective To investigate the apoptosis effect induced by D limonene on BGC 823 gastric cancer cells. Methods The expression of p53, bc1 2 in BGC 823 cells and qualitative, quantitative index of cell apoptosis were detected with MTT, electron microscopy, flow cytometry and immunohistochemical method. ResultsD limonene could induce the formation of apoptotic bodies in a dose and time dependent manner. The expression of bcl 2 protein decreased and p53 protein increased in BGC 823 cells treated with D limonene, compared with the control cells. Conclusion D limonene exerts its cytotoxic effect on BGC 823 gastric cancer cells by inducing apoptosis. [ |
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| Keywords: | Stomach neoplasms D limonene Apoptosis BGC 823 cell line |
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