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Draft genome sequence of plastic degrading Bacillus sp. AIIW2 isolated from the Arabian ocean
Authors:Alka Kumari  Nasreen Bano  Doongar R. Chaudhary  Bhavanath Jha
Affiliation:1. Division of Plant Omics, CSIR-Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, India;2. Department of Molecular Biology and Biotechnology, CSIR-National Botanical Research Institute, Lucknow, Uttar Pradesh, India

CSIR-Academy of Scientific and Innovative Research, Ghaziabad, Uttar Pradesh, India

Abstract:The endemic spread of plastic in the environment requires urgent need of a sustainable approach. Marine microbes found to have vast bioactivity and play a central role in biogeochemical cycling in the ocean; however, very few of them had been explored for biochemical cycling or plastic degradation. In the present study, we report the draft genome sequence of marine Bacillus sp. AIIW2 which was found to utilize plastic as a carbon source. The Bacillus sonorensis SRCM101395 was used as a reference genome for mapping the reads. The genome size of strain AIIW2 was approximately 4.4 Mb and composed of 4737 coding sequences with 45.7% G + C contents. The whole genome comparison of strain AIIW2 with three closest Bacillus strains showed strain specificity, the 16S ribosomal RNA sequence shows 99.93% similarity with Bacillus paralicheniformis KJ-16T (KY694465). This genome data would provide the genetic basis in developing plastic bioremediation approaches and discover the enzymes pertinent in the biodegradation processes.
Keywords:biodegradation  draft genome  marine bacteria  plastic
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