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Endophytic colonization of entomopathogenic fungi increases plant disease resistance by changing the endophytic bacterial community |
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| Authors: | Yuming Chang Xinyao Xia Li Sui Qin Kang Yang Lu Le Li Wende Liu Qiyun Li Zhengkun Zhang |
| Affiliation: | 1. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, China College of Life Sciences, Jilin Agricultural University, Changchun, China;2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing, China;3. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, China;4. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, China Department of Entomology, College of Plant Protection, China Agricultural University, Beijing, China;5. Jilin Key Laboratory of Agricultural Microbiology, Key Laboratory of Integrated Pest Management on Crops in Northeast China, Ministry of Agriculture and Rural Affairs, Jilin Academy of Agricultural Sciences, Changchun, China College of Plant Protection, Jilin Agricultural University, Changchun, China |
| Abstract: | Various mechanisms are involved in plant disease resistance mediated by entomopathogenic fungi; however, the role of plant endophytic microbes in disease resistance is unknown. In the present study, we showed that the disease incidence of northern corn leaf blight caused by Exserohilum turcicum (Et) on maize was reduced significantly by soil inoculation with Beauveria bassiana (Bb). Meanwhile, B. bassiana colonization and E. turcicum infection increased the diversity and abundance and diversity of endophytic bacteria and fungi, respectively, while the abundance of endophytic bacterial of the Bb + Et treatment decreased significantly compared with that of Et treatment alone. However, Bb + Et treatment increased the relative abundance of plant beneficial bacteria significantly, for example, Burkholderia and Pseudomonas. Network analyses showed that the microbiome complexity increased after soil inoculation with B. bassiana. Taken together, these results revealed the potential mechanism by which entomopathogenic fungi exert biological control of maize leaf spot disease. |
| Keywords: | Beauveria bassiana biocontrol endophytic microbiome Exserohilum turcicum fungal endophyte maize |