抑制EBNA1表达对NK/T细胞淋巴瘤细胞增殖的影响

目的 研究抑制EBV阳性NK/T细胞淋巴瘤细胞系HANK1细胞EBV核抗原1的表达对细胞增殖和周期分布的影响，探讨抑制EBV感染治疗EBV相关性NK/T细胞淋巴瘤的潜在应用价值。方法 用RNA干扰（RNA interference，RNAi）方法抑制HANK1细胞EBNA1表达，Western blot检测EBNA1蛋白表达，流式细胞仪检测细胞周期分布和细胞凋亡。结果EBNA1-shRNA表达载体明显抑制EBNA1表达，细胞周期G₁期停滞，凋亡细胞增多，细胞增殖受抑。结论 根治EBV感染可能是治疗EBV相关性NK/T细胞淋巴瘤的好方法。

Influence of Silencing EBNA1 Using RNAi Strategy on Cell Proliferation of NK/T Cell Lymphoma Cells

Objective To study the cell proliferation and cell cycle distributions of EBV-positive NK/T cell lymphoma cell line (HANK1 cells) after silencing their EBNA1 expression, and to investigate the potential role of elimination of EBV infection on the treatment of EBV-associated NK/T cell lymphoma. Methods EBNA1 expression was silenced in HANK1 cells using RNA interference (RNAi) strategy, and the expression of EBNA1 was evaluated by means of Western blot.The cell cycle distributions and apoptosis were detected using flow cytometry. Results Suppressing EBNA1 expression caused cell cycle arrested at G1 phase, induced cell apoptosis and inhibited cell proliferation in EBV-positive HANK1 cells. Conclusion Elimination of EBV infection may be a good therapy for patients with EBV-associated NK/T cell lymphomas.