| 红霉素链霉菌遗传控制的研究 2.红霉素链霉菌耐前体耐红霉素高产菌株的筛选 |
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| 引用本文： | 熊宗贵,陈桂琴,袁杰利,陈淑华.红霉素链霉菌遗传控制的研究 2.红霉素链霉菌耐前体耐红霉素高产菌株的筛选[J].沈阳药科大学学报,1985(4). |
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| 作者姓名： | 熊宗贵 陈桂琴 袁杰利 陈淑华 |
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| 作者单位： | 沈阳药学院抗生素教研室，沈阳药学院抗生素教研室，沈阳药学院抗生素教研室，沈阳药学院抗生素教研室 沈阳药学院微生物制药专业84届毕业生，沈阳药学院微生物制药专业84届毕业生 |
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| 摘 要： | 利用长波紫外线和8-甲氧基补骨脂素光敏化物质诱变处理红霉素链霉菌14-74,在含有红霉素或和前体的培养基中,筛选得到了红霉素高产变株。在含有红霉素2万μg/ml 的种子培养基中,筛得活性提高6.2%的耐性变株;在含有6%正丙醇的种子培养基中,得到活性提高12.4%的耐性变株。将耐红霉素3万μg/ml 的变株进一步用长波紫外线诱变处理,在含有5%正丙醇的种子培养基中进行筛选,得到耐红霉素和正丙醇,活性提高19.1%的变株。
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| 关 键 词： | 红霉素链霉菌 诱变 抗性变株 红霉素 |
STUDIES ON GENETIC MANIPULATION OF STREPTOMYCES ERYTHREUS 2.Selection of the high yielding strains of S.erythreus which are precursor-resistant and erythromycin-resistant mutants |
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| Xiong Zonggui Chen Guiqin Yuan Jieli Chen Shuhua.STUDIES ON GENETIC MANIPULATION OF STREPTOMYCES ERYTHREUS 2.Selection of the high yielding strains of S.erythreus which are precursor-resistant and erythromycin-resistant mutants[J].Journal of Shenyang Pharmaceutical University,1985(4). |
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| Authors: | Xiong Zonggui Chen Guiqin Yuan Jieli Chen Shuhua |
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| Institution: | Xiong Zonggui Chen Guiqin Yuan Jieli Chen Shuhua Department of Antibiotics |
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| Abstract: | Through the mutation of using near UV and 8-methoxypsoralen to treat S. erythreus 14—74,the high yielding strains were selected in the medium contain- ing erythromycin or/and precursor from resistant mutants.In the inoculum medium containing 20,000μg/ml of erythromycin,the resistant mutant showing 6.2% higher activity was isolated and containing 6% n-propanol,so was the resistant mutant showing 12.4% higher activity.The mutant resistant 30,000μg/ml of erythromycin was further treated by near UV,the mutant showing 19.1% higher activity was isolated in the medium containing 5% n-propanol. |
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| Keywords: | Streptomyces erythreus Mutation Resistant mutant Erythromycin |
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