| 针刺对大鼠脊髓损伤后内源性神经干细胞的影响 |
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| 引用本文： | 刘丽霞,邰浩清,常加松,王长明. 针刺对大鼠脊髓损伤后内源性神经干细胞的影响[J]. 中国中医急症, 2011, 20(2): 270-272 |
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| 作者姓名： | 刘丽霞 邰浩清 常加松 王长明 |
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| 作者单位： | 南京中医药大学,江苏,南京,210046 |
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| 摘 要： | 目的探讨针刺对大鼠脊髓损伤后内源性神经干细胞（ENSCs）的诱导分化。方法 25只成年SD雄性大鼠,随机分为假手术组、模型组和针刺治疗组。假手术组不损伤脊髓,模型组和针刺治疗组用改良Allen’s重物坠击法制备脊髓损伤模型。模型组不予任何处理;针刺治疗组予以针刺治疗,每日1次,7d为1疗程,共2个疗程。于2周后取材,对距离损伤中心5mm的脊髓行免疫组化染色,检测Nestin、Brdu、NSE、GFAP的表达。结果 Nestin和Brdu的表达针刺治疗组较模型组多,假手术组最少;NSE的表达针刺治疗组较模型组多,但少于假手术组;GFAP的表达模型组高于针刺治疗组,假手术组最低。结论针刺对大鼠脊髓损伤后内源性神经干细胞有诱导分化作用。
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| 关 键 词： | 脊髓损伤 针刺 内源性神经干细胞 大鼠 |
Effect of Acupuncture on the Endogenous Neural Stem Cells of Rats with Spinal Cord Injury |
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| LIU Li-xia,TAI Hao-qing,CHANG Jia-song,et al Nanjing University of Chinese Medicine. Effect of Acupuncture on the Endogenous Neural Stem Cells of Rats with Spinal Cord Injury[J]. Journal of Emergency in Traditional Chinese Medicine, 2011, 20(2): 270-272 |
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| Authors: | LIU Li-xia TAI Hao-qing CHANG Jia-song et al Nanjing University of Chinese Medicine |
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| Affiliation: | LIU Li-xia,TAI Hao-qing,CHANG Jia-song,et al Nanjing University of Chinese Medicine(Nanjing,Jiangsu 210046) |
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| Abstract: | Objective:To investigate the differentiation effect of acupuncture on endogenous neural stem cells(ENSCs) after spinal cord injury in rats.Methods:25 SD rats were randomly divided into 3 groups such as the sham-operation group,the model group and the acupuncture group.The Allen's method was used to be the spinal cord injury model.After 2 treatment courses,the spinal cords were stained by the immunohistochemical method.And then,expressions of Nestin,Brdu,NSE and GFAP were tested.Results:The Nestin and the Br... |
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| Keywords: | Spinal cord injury Acupuncture Endogenous neural stem cells Rats |
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