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| 蛇六谷乙酸乙酯萃取物A_1组分对大鼠胶质瘤C_6细胞增殖及C-myc、bcl-2、p~（53）癌基因表达的影响 | |
| 引用本文： | 陈培丰,常中飞,丁志山,吴巧凤,胡秀敏. 蛇六谷乙酸乙酯萃取物A_1组分对大鼠胶质瘤C_6细胞增殖及C-myc、bcl-2、p~（53）癌基因表达的影响[J]. 中国中医药科技, 2011, 18(1): 28-30,95 |
| 作者姓名： | 陈培丰 常中飞 丁志山 吴巧凤 胡秀敏 |
| 作者单位： | 1. 浙江中医药大学附属第一医院·杭州,310006 2. 北京石景山医院·北京,100043 3. 浙江中医药大学·杭州,310053 |
| 基金项目： | 浙江省自然科学基金课题 |
| 摘 要： | 目的：探讨蛇六谷乙酸乙酯萃取物A1组分对大鼠胶质瘤C6细胞增殖的抑制作用及其作用机制。方法：用MTT比色法检测不同浓度A1组分对C6细胞增殖的抑制作用,RT-PCR法检测A1组分对C6细胞C-myc、bc l-2,p53mRNA基因表达的影响。结果：A1组分对C6细胞的增殖具有明显的抑制作用,且呈量-效关系,并有明显的细胞形态学改变;RT-PCR结果显示,随着A1组分作用剂量的增加,C6细胞的C-myc、bc l-2及p53mRNA表达均有明显降低。结论：蛇六谷乙酸乙酯萃取物A1组分对大鼠胶质瘤C6细胞的增殖具有明显的抑制作用,其作用机制可能与抑制原癌基因C-myc、bc l-2及p53的表达有关。 |
| 关 键 词： | 蛇六谷乙酸乙酯萃取物A1/药理学 胶质瘤C6细胞/药物作用 体外研究 |
Effects of A1 Component of Konjac Ethyl Acetate Extract on Multiplication of Rats' Gliocytoma C6 Cells and C-myc,Bcl-2 and P53 Expression |
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| CHEN Peifeng,CHANG Zhongfei,DING Zhishan,et al. Effects of A1 Component of Konjac Ethyl Acetate Extract on Multiplication of Rats' Gliocytoma C6 Cells and C-myc,Bcl-2 and P53 Expression[J]. Chinese Journal of Traditional Medical Science and Technology, 2011, 18(1): 28-30,95 | |
| Authors: | CHEN Peifeng CHANG Zhongfei DING Zhishan et al |
| Affiliation: | CHEN Peifeng,CHANG Zhongfei,DING Zhishan,et al(The First Hospital Affiliated to Zhejiang University of TCM,Hangzhou310006) |
| Abstract: | Objective: To observe the inhibition of A1 component of konjac ethyl acetate extract on multiplication of rats' gliocytoma C6 cells and explore the mechanism.Methods: The inhibition of A1component of konjac ethyl acetate extract on multiplication of rats' gliocytoma C6 cells was determined by MTT method,the effect of A1component of konjac ethyl acetate extract on C-myc,bcl-2 and p53 expression in rats' gliocytoma C6 cells were tested by RT-PCR.Results: A1 component of konjac ethyl acetate extract could obvi... |
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