| Caveolin-1对内皮细胞增殖的抑制作用研究 |
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| 引用本文： | 房凯,刘军. Caveolin-1对内皮细胞增殖的抑制作用研究[J]. 中华肿瘤防治杂志, 2007, 14(9): 660-661,685 |
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| 作者姓名： | 房凯 刘军 |
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| 作者单位： | 中国医科大学基础医学院生物信息学教研室,辽宁,沈阳,110001;中国医科大学基础医学院生物信息学教研室,辽宁,沈阳,110001 |
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| 摘 要： | 目的：研究Caveolin-1蛋白在内皮细胞增殖中的作用,其与Ras-p42/44MAPK细胞信号通路的关系.方法：以腺病毒介导在内皮细胞中表达外源Caveolin-1蛋白,分析其对内皮细胞增殖和p42/44MAPK分子磷酸化水平的影响.结果：感染腺病毒表达外源Caveolin-1蛋白的内皮细胞中,血管内皮生长因子（VEGF）诱导的3H-胸腺嘧啶掺入量降低了35%（n=3,P=0.009）,p42/44MAPK分子磷酸化水平降低了47.6%.结论：Caveolin-1蛋白抑制p42/44MAPK分子磷酸化水平,抑制内皮细胞增殖.
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| 关 键 词： | 肿瘤/病理学 肿瘤蛋白质类 内皮 血管/细胞学 血管生成抑制剂 |
| 文章编号： | 1673-5269（2007）09-0660-02 |
| 收稿时间： | 2006-10-11 |
| 修稿时间： | 2007-01-19 |
Effect of caveolin-1 on endothelial cells proliferation |
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| FANG Kai,LIU Jun. Effect of caveolin-1 on endothelial cells proliferation[J]. Chinese Journal of Cancer Prevention and Treatment, 2007, 14(9): 660-661,685 |
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| Authors: | FANG Kai LIU Jun |
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| Abstract: | OBJECTIVE:To investigate the effect of caveolin-1 on the endothelial cell proliferation and the molecular mechanism.METHODS:The adenovirus as a vector was exployed to express the exogenous caveolin-1 protein,the proliferation by was determined 3H-thymidine incorporation assay,and the activity of p42/44MAPK was determined by Western blot.RESULTS:The incorporation of 3H-thymidine induced by VEGF was decreased by 35%(n=3,P=0.009)in endothelial cells,and the phosphorylation level of p42/44MAPK was decreased by 47.6%.CONCLUSION:Caveolin-1 inhibits the phosphorylation level of p42/44MAPK and the proliferation of endothelial cells. |
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| Keywords: | neoplasms/pathology neoplasm proteins endothelium, vascular/cytology angiogenesis inhibitors |
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