| 黄曲霉毒素B_1人工抗原及抗体的制备研究 |
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| 引用本文： | 李卫岗,鲁长豪,肖志芳,王光建. 黄曲霉毒素B_1人工抗原及抗体的制备研究[J]. 四川大学学报(医学版), 1992, 0(3) |
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| 作者姓名： | 李卫岗 鲁长豪 肖志芳 王光建 |
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| 作者单位： | 华西医大卫生检测教研室(李卫岗，鲁长豪，肖志芳)，华西医大卫生检测教研室(王光建) |
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| 摘 要： | 作者用黄曲霉毒素B_1(AFB_1)与羧甲基羟胺半盐酸盐(CMO)在碱性介质中回流反应,使AFB_1生成AFB_1羧甲基肟(AFB_1-oxime),将此衍生物偶联到牛血清白蛋白(BSA)上,制得人工抗原AFB_1-oxime-BSA。该人工抗原经纯化后免疫家兔,获得高效价(1:100 000)、高特异性的抗AFB_1的抗体,可供用于免疫分析法测定AFB_1的含量。
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| 关 键 词： | 黄曲霉毒素B_1 人工抗原 抗体 兔 |
Preparation or Antigen and Antibody Against Aflatoxin B_1 |
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| Li Weigang,Lu Changhao,Xiao Zhifang,Wang Guangjian. Preparation or Antigen and Antibody Against Aflatoxin B_1[J]. Journal of Sichuan University. Medical science edition, 1992, 0(3) |
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| Authors: | Li Weigang Lu Changhao Xiao Zhifang Wang Guangjian |
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| Affiliation: | Li Weigang;Lu Changhao;Xiao Zhifang;Wang Guangjian Department of Sanitary Technology |
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| Abstract: | Carboxymathyloxime of afla-toxin B_1 (AFB_1)was synthesized by refluxingAF B_1 with carboxymethoxylamine hemi-hydrochloride for 4 hours in the presenceof pyridine. AF B_1-BSA(antigen) was madesuccessfully by coupling AF B_1-oxime withbovine serum albumin(BSA) in the presenceof a water soluble carbodiimide. The rab-bits were immunized with the artificialimmunogen which had been purified by di-alysis. The titers of antisera were dater-mined by indirect enzyme-linked immuno-sorbant assay (ELISA) which was develo-ped by ourselves. The highest titer was1: 100 000. |
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| Keywords: | Aflatoxin B_1 Antigen Antibody Rabbit |
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