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| SNL大鼠CD4+ T细胞脊髓浸润及其分子机制研究 | |
| 引用本文： | 孙彩霞,刘嫣方,周成林,陈盼,张盼,苏兆亮,朱琛平,王胜军,许化溪.SNL大鼠CD4+ T细胞脊髓浸润及其分子机制研究[J].细胞与分子免疫学杂志,2011,27(12):1291-1294. |
| 作者姓名： | 孙彩霞 刘嫣方 周成林 陈盼 张盼 苏兆亮 朱琛平 王胜军 许化溪 |
| 作者单位： | 1. 江苏大学附属医院麻醉科;江苏大学免疫学研究所,江苏镇江212013 2. 镇江出入境检验检疫局 3. 江苏大学附属医院麻醉科 4. 江苏大学免疫学研究所,江苏镇江,212013 5. 镇江市京口社区卫生服务中心,江苏镇江,212001 |
| 基金项目： | 国家自然科学基金资助(81001319); 镇江市社会发展项目(SH2011028); 江苏大学临床医学科技发展基金资助(JLY2010135) |
| 摘 要： | 目的:研究脊神经结扎后,外周CD4+ T细胞迁移浸润至腰段脊髓及其分子调控机制.方法:选择健康成年清洁级雄性SD大鼠,随机分为脊神经结扎组(Tx 组)、假冒手术组(S组)、对照组(C组),用up-down方法测定50％机械缩足阈值(50％ MWT),并用FACS检测腰段脊髓CD4+ T细胞的浸润情况,RT-qPCR法测定脊髓中趋化因子CCL2、CCL5、CXCL10mRNA的表达水平,ELISA检测血清中相关细胞因子的含量.结果:与S组、C组相比,术后3 d,Tx组50％ MWT明显降低(P＜0.01),至术后14 d达最低值,S组与C组在各时间点均无明显差异(P＞0.05);术后7d,Tx组腰段脊髓浸润的CD4+ T细胞明显增加(P＜0.01),同时伴有CCL2、CCL5mRNA 的表达增加(P＜0.05);术后14 d,Tx组浸润的CD4+T 细胞较术后7d减少,虽高于S组、C组,但无统计学意义,上述各趋化因子的表达水平也无明显差异;术后7d、14 d,血清中各细胞因子的含量三组均无统计学意义.结论:神经损伤后,脊髓中高表达的趋化因子促进了外周CD4+ T细胞的中枢浸润,脊髓浸润的CD4+T 细胞可能与神经病理性疼痛的维持有关. |
| 关 键 词： | 神经病理性疼痛 趋化因子 CD4+T细胞 |
The pathogenesis of CD4(+)T cells infiltrated into the spinal cord in rat SNL model |
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| SUN Cai-xia , LIU Yah-fang , ZHOU Cheng-lin , CHEN Pan , ZHANG Pan , SU Zhao-liang , ZHU Chen-ping , WANG Sheng-jun , XU Hua-xi.The pathogenesis of CD4(+)T cells infiltrated into the spinal cord in rat SNL model[J].Journal of Cellular and Molecular Immunology,2011,27(12):1291-1294. | |
| Authors: | SUN Cai-xia LIU Yah-fang ZHOU Cheng-lin CHEN Pan ZHANG Pan SU Zhao-liang ZHU Chen-ping WANG Sheng-jun XU Hua-xi |
| Institution: | SUN Cai-xia1,2,LIU Yan-fang1,ZHOU Cheng-lin3,CHEN Pan1,ZHANG Pan1,SU Zhao-liang2,ZHU Chen-ping4,WANG Sheng-jun2,XU Hua-xi2 1Department of Anesthesiology,the Affiliated Hospital of Jiangsu University,2Department of Immunology,Jiangsu University,Zhenjiang 212013,3Zhenjiang Entry-exit Inspection and Quarantine Bureau,4Community Health Service Center of Zhenjiang,Zhenjiang 212001,China |
| Abstract: | AIM: To explore the infiltration pathogenesis of CD4+T cells following the spinal nerve ligation.METHODS: Healthy adult male SD rats were randomly divided into the spinal nerve ligation group(Tx),sham operation group(S),control group(C).the 50% mechanical paw withdrawal threshold(50%MWT) was determined by up-down method;CD4+T cells infiltration was assessed by FACS;the mRNA levels of CCL2,CCL5 and CXCL10 were quantitated by RT-qPCR;serum cytokines were tested by ELISA kits.RESULTS: After 3 days since operat... |
| Keywords: | neuropathic pain chemokine CD4 T cell |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |